Evaluation of the Ophthalmotoxic Effect of Quantum Dots and Bioconjugates Based on Them in Terms of the Prospects for the Treatment of Resistant Endophthalmitis. Experimental Research (Stage 1)
Evaluation of the Ophthalmotoxic Effect of Quantum Dots and Bioconjugates Based on Them in Terms of the Prospects for the Treatment of Resistant Endophthalmitis. Experimental Research (Stage 1)
复制标题
量子点和基于它们的生物缀合物的眼毒性作用评价对治疗耐药性眼内炎的前景。
DOI：
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
S. Dezhurov
中科院分区：
文献类型：
--
作者：
V. O. Ponomarev;V. Kazaykin;A. V. Lizunov;A. Vokhmintsev;I. A. Vainshtein;S. Dezhurov
眼内炎仍然是眼科手术中最可怕的并发症之一，导致显著的功能和解剖变化。治疗这种病理的“黄金”标准是安装静脉注射抗菌药物，但考虑到病原体的数量和类型，抗生素耐药性的增长，寻找治疗眼内炎的替代方法仍然是相关的。胶体量子点是纳米级半导体晶体，由于其体积，成分和表面连接的变化而具有模拟的光学和电子特性，因此对该方向的研究感兴趣。本文介绍了CT及其生物缀合物的合成过程，以评估其眼毒性，并展望其在眼内炎治疗中的应用前景。该研究分为4个阶段，首先确定所需的技术规格，以便考虑到物理和化学特性选择适当类型的量子点（阶段1），量子点的合成（阶段2），用于植入玻璃体腔的各种浓度的量子点溶液的制备和滴定（阶段3）。最后阶段是评估当在动物模型上玻璃体内施用时，量子点溶液以其纯形式以及与抗生素（头孢他啶和万古霉素）组合的毒性作用。作为研究的结果，合成了量子点，并获得了基于它们的溶液，用于引入玻璃体腔。根据动物模型（家兔）的试验，确定了该溶液的安全剂量，以及其与抗生素联合使用的可能性。
Endophthalmitis remains one of the most formidable complications of surgery in ophthalmology, leading to significant functional and anatomical changes. The “gold” standard of treatment for this pathology is the installation of intravenous injections of antibacterial drugs, but taking into account the number and type of pathogens, the growth of antibiotic resistance, the search for alternative methods of treatment of endophthalmitis remains relevant. Colloidal quantum dots, which are nanoscale semiconductor crystals with simulated optical and electronic properties due to changes in their volume, composition, and surface connections, are of interest for research in this direction. This article presents the process of synthesis of CT and bioconjugates based on them in order to assess ophthalmotoxicity with the prospect of further use in the treatment of endophthalmitis. The study was divided into 4 stages, starting with the determination of the required technical specification in order to select the appropriate type of quantum dots taking into account the physical and chemical characteristics (Stage 1), the synthesis of quantum dots (Stage 2), the preparation and titration of a solution of quantum dots of various concentrations for implantation in the vitreous cavity (Stage 3). The final stage was to evaluate the toxic effect of the quantum dot solution in its pure form, as well as in combination with antibiotics (ceftazidime and vancomycin) when administered intravitreally on an animal model. As a result of the study, quantum dots were synthesized and a solution based on them was obtained for introduction into the vitreous cavity. Based on the testing of the animal model (rabbits), a safe dose of the solution was determined, as well as the possibility of its use in combination with antibiotics.
影响因子：
4.2
作者：
Rao, NA;Hidayat, AA
通讯作者：
Hidayat, AA