Stable carbon isotope analyses of nanogram quantities of particulate organic carbon (pollen) with laser ablation nano combustion gas chromatography/isotope ratio mass spectrometry
Stable carbon isotope analyses of nanogram quantities of particulate organic carbon (pollen) with laser ablation nano combustion gas chromatography/isotope ratio mass spectrometry
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使用激光烧蚀纳米燃烧气相色谱/同位素比质谱法对纳克级颗粒有机碳（花粉）进行稳定碳同位素分析
DOI：
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发表时间：
2016
影响因子：
2
通讯作者：
G. Reichart
中科院分区：
文献类型：
--
作者：
Linda van Roij;A. Sluijs;Jelmer Laks;G. Reichart
有机和无机物质残留物的稳定碳同位素比率(δ13C值)的基本原理分析有助于我们对现在和过去的环境和生物过程的大部分了解。直到最近，这种分析的分析窗口一直局限于含有至少几个μg碳的样品。方法采用激光消融、纳米燃烧气相色谱和同位素比值质谱仪(LA/NC/GC/IRMS)相结合的方法。与以前的研究相比，使用深紫外光(193 Nm)激光以最小的分馏效应优化有机物的粉碎，并使用非常小的烧蚀室和燃烧炉来降低最小样品质量要求。结果对国际原子能机构CH-7聚乙烯标准的分析表明，当测量至少42 ng C时，准确度最佳，精密度优于0.5ngC。应用于未经处理的现代蓝桉(C3植物)和玉米(C4植物)花粉粒，这两个物种之间的偏移量为16‰。在每一粒玉米花粉粒内，重复分析显示δ13C值几乎相同。结论个体花粉粒之间的同位素偏移量超出了分析的不确定性，因此可能反映了样品间约0.5-0.9‰的变异性。这些有希望的结果为研究δ13C值和自然碳同位素变异性奠定了基础，这些样品是由产生数十毫微克碳的各种有机颗粒组成的单一种群的单个样本。©2016作者。《质谱学中的快速通信》，John Wiley&Sons Ltd.出版。
Rationale Analyses of stable carbon isotope ratios (δ 13C values) of organic and inorganic matter remains have been instrumental for much of our understanding of present and past environmental and biological processes. Until recently, the analytical window of such analyses has been limited to samples containing at least several μg of carbon. Methods Here we present a setup combining laser ablation, nano combustion gas chromatography and isotope ratio mass spectrometry (LA/nC/GC/IRMS). A deep UV (193 nm) laser is used for optimal fragmentation of organic matter with minimum fractionation effects and an exceptionally small ablation chamber and combustion oven are used to reduce the minimum sample mass requirement compared with previous studies. Results Analyses of the international IAEA CH‐7 polyethylene standard show optimal accuracy, and precision better than 0.5‰, when measuring at least 42 ng C. Application to untreated modern Eucalyptus globulus (C3 plant) and Zea mays (C4 plant) pollen grains shows a ~ 16‰ offset between these species. Within each single Z. mays pollen grain, replicate analyses show almost identical δ 13C values. Conclusions Isotopic offsets between individual pollen grains exceed analytical uncertainties, therefore probably reflecting interspecimen variability of ~0.5–0.9‰. These promising results set the stage for investigating both δ 13C values and natural carbon isotopic variability between single specimens of a single population of all kinds of organic particles yielding tens of nanograms of carbon. © 2016 The Authors. Rapid Communications in Mass Spectrometry Published by John Wiley & Sons Ltd.
影响因子：
5
作者：
A. Pearson;Sarah J. Hurley;S. R. S. Walter-S.-R.-S.-Walter-39267913;S. Kusch;S. Lichtin;Yi Ge Zhang
通讯作者：
A. Pearson;Sarah J. Hurley;S. R. S. Walter-S.-R.-S.-Walter-39267913;S. Kusch;S. Lichtin;Yi Ge Zhang
一种耦合高效液相色谱和化学反应界面质谱的移动带装置。
DOI：
10.1002/bms.1200200512
发表时间：
1991
期刊：
Biological mass spectrometry
影响因子：
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作者：
Moini,M;Abramson,FP
通讯作者：
Abramson,FP