Permeability of the diaphragmatic mesothelium: the ultrastructural basis for "stomata".
Permeability of the diaphragmatic mesothelium: the ultrastructural basis for "stomata".
复制标题
膈间皮的渗透性：“气孔”的超微结构基础。
DOI：
10.1002/aja.1001510409
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
K. Rahil
中科院分区：
文献类型：
--
作者：
L. V. Leak;K. Rahil
本文用透射电镜、扫描电镜和冷冻断裂复制技术研究了正常小鼠和腹腔注射小鼠膈肌腹膜表面细胞的组织结构。隔膜腹膜表面的间皮细胞被组织成两个离散的群体。在淋巴陷窝上方的区域（覆盖中皮下淋巴管的屋顶）发现了一群立方细胞，而隔膜的其余区域（不覆盖淋巴陷窝）则由非常扁平的细胞排列。立方形和扁平的间皮细胞的顶端表面覆盖着大量的微绒毛。立方细胞的侧边界的特征在于存在许多丝状突起，这些丝状突起与相邻细胞的类似结构交错，形成细胞间间隙，这些间隙覆盖在结缔组织的薄层上。除了细胞间隙外，在几个相邻细胞的边缘之间还发现了特殊的圆孔。这些观察结果表明，这些圆形孔形成时，几个淋巴管内皮细胞的细胞边缘跨越亚mesothelium结缔组织形成细胞间连接与细胞边缘的几个相邻的mesothelium细胞内衬腹膜表面的隔膜。这两种不同细胞类型之间的密切细胞间接触提供了腹膜腔和腹膜淋巴管腔（淋巴陷窝）之间的开放通道。这表明，这些圆形孔对应于冯雷克林豪森的“气孔”。腹腔注射胶体。1799年左右William Hunter对浆膜腔淋巴管生理学的研究表明，当注入腹膜腔和胸膜腔时，液体、颗粒物质和细胞被迅速吸收（Hewson，1846）。还观察到含有活体染料的水和盐溶液比颗粒物质更快地被吸附（Starling和Tubby，1894）。随后对哺乳动物和两栖动物胸膜腔和腹膜腔中液体和payticulate物质吸收的研究表明，腹膜腔是淋巴系统的复杂部分，吸收主要通过腹膜下淋巴管进行。然而，直到Heidenhain（1895）关于分泌的工作，
The topograplhy and organization of cells lining the peritoneal surface of the diaphragm in inormal and intraperitoneally injected mice have been studied by transmission and scanning electron microscopy and freeze-fracture replication techniques. The mesothelid cells of the peritoneal surface of the diaphragm are organized into two discrete populations. A population of cuboidal cells are found in areas over the lymphatic lacuna (roof covering submesothelial lymphatic vessels) while the remaining areas (not covering lymphatic lacunae) of the diaphragm are lined by extremely flattened cells. The apical surfaces of both cuboidal and flattened mesothelial cells are covered with numerous microvilli. The lateral borders of the cuboidal cells are characterized by the presence of numerous filamentous processes which interlace with similar structures from adjacent cells to form intercellular gaps which overlie a thin layer of connective tissue. In addition to the intercellular gap: $, special circular pores are found between the margins of several neighboring cells. The observations suggest that these circular pores are formed when cell margins of several lymphatic endothelial cells span the submesothelial connective tissue to form intercellular junctions with cell margins of several neighboring mesothelial cells lining the peritoneal surface of the diaphragm. The intimate intercellular contact between these two different cell types provides an open channel between the peritoneal cavity and the lumen of the diaphragmatic lymphatic vessels (lymphatic lacunae). It is suggested that these circular pores correspond to the “stomata” of von Recklinghausen. Intraperitoneally injected col1oida. l particles (biological carbon) and red blood cells are rapidly removed from the peritoneal cavity via the circular pores (stomata).The studies of William Hunter, around 1799, on the physiology of the lymphatic vessels of the serous cavities demonstrated that fluids and particulate substances and cells were rapidly absorbed when injected into the peritoneal and pleural cavities (Hewson, 1846). It was also observed that water and salt solutions containing vital dyes were iibsorbed more rapidly than particulate substances (Starling and Tubby, 1894). Subsequent studies on the absorption of fluids and payticulate substances from both pleural and peritoneal cavities of mammals and amphibians suggested that the peritoneal cavity is an intricate part of the lymphatic system with absorption taking place primarily by the subperitoneal lymphatics. However, it was not until the work of Heidenhain (1895) on the secretion of