High levels of foreign gene expression in hepatocytes after tail vein injections of naked plasmid DNA
High levels of foreign gene expression in hepatocytes after tail vein injections of naked plasmid DNA
复制标题
DOI：
10.1089/10430349950017734
复制
发表时间：
1999-07-01
影响因子：
4.2
通讯作者：
Wolff, JA
中科院分区：
文献类型：
--
作者：
Zhang, GF;Budker, V;Wolff, JA
我们以前已经表明，肌肉注射裸质粒DNA使外源基因在肌肉中表达。进一步的研究表明，裸质粒DNA的血管内递送不仅能够在肌肉中而且能够在肝细胞中实现高水平的表达。对于肝脏，该技术需要直接注射到肝血管（门静脉、肝静脉或胆管）中并阻断流出。目前的研究表明，肝细胞中的高水平的质粒DNA表达可以很容易地通过尾静脉注射获得。最高水平的表达通过快速注射大体积的质粒DNA来实现，类似于2.5 ml。该技术具有广泛的实验室研究的巨大潜力。
We have previously shown that the intramuscular injection of naked plasmid DNA enables foreign gene expression in muscle. Further studies showed that the intravascular delivery of naked plasmid DNA enables high levels of expression not only in muscle but also in hepatocytes. For the liver, this technique required injection directly into the liver vessels (portal vein, hepatic vein, or bile duct) and occlusion of outflow. The present study now demonstrates that high levels of plasmid DNA expression in hepatocytes can be easily obtained by tail vein injections. The highest levels of expression are achieved by rapidly injecting the plasmid DNA in large volumes, similar to 2.5 ml. This technique has great potential for a wide variety of laboratory studies.