ISOLATION AND EXPRESSION OF A CDNA-ENCODING THE PRECURSOR FOR A NOVEL MEMBER (ACADSB) OF THE ACYL-COA DEHYDROGENASE GENE FAMILY
ISOLATION AND EXPRESSION OF A CDNA-ENCODING THE PRECURSOR FOR A NOVEL MEMBER (ACADSB) OF THE ACYL-COA DEHYDROGENASE GENE FAMILY
复制标题
DOI：
10.1006/geno.1994.1617
复制
发表时间：
1994-11-15
期刊：
影响因子：
4.4
通讯作者：
FOURNIER, B
中科院分区：
文献类型：
--
作者：
ROZEN, R;VOCKLEY, J;FOURNIER, B
酰基辅酶a脱氢酶（ACDs）是在脂肪酸或支链氨基酸代谢过程中氧化直链或支链酰基辅酶a的线粒体酶家族。该基因家族成员的缺失是人类疾病的重要原因。编码ACD基因家族新成员（基因符号ACADSB）的人类前体cDNA已被分离并鉴定。1.3 kb的开放阅读框编码431个氨基酸的前体蛋白，体外加工产生399个氨基酸的成熟蛋白。该cDNA与其他acyl-CoA脱氢酶家族成员具有显著的序列相似性，与短链acyl-CoA脱氢酶的同源性最高（38%）。cDNA在真核细胞（COS）和原核细胞（大肠杆菌）中表达，产生了预期大小的蛋白，对短支链酰基辅酶a衍生物（(S)-2甲基丁基辅酶a、异丁基辅酶a和2-甲基己醇基辅酶a）以及短直链酰基辅酶a（丁基辅酶a和己醇基辅酶a）具有活性。(C) 1994学术出版社，Inc.
The acyl-CoA dehydrogenases (ACDs) are a family of mitochondrial enzymes that oxidize straight chain or branched chain acyl-CoAs in the metabolism of fatty acids or branched chain amino acids. Deficiencies in members of this gene family are important causes of human disease. A cDNA encoding the human precursor for a novel member (gene symbol ACADSB) of the ACD gene family has been isolated and characterized. The open reading frame of 1.3 kb encodes a precursor protein of 431 amino acids, which is processed in vitro to yield a mature protein of 399 amino acids. The cDNA has significant sequence similarity to other members of the acyl-CoA dehydrogenase family, with the greatest homology (38%) to the short chain acyl-CoA dehydrogenase. The cDNA was expressed in eukaryotic (COS) and prokaryotic (Escherichia coli) cells, producing a protein of the expected size, with activity toward the short branched chain acyl-CoA derivatives ((S)-2methylbutyryl-CoA, isobutyryl-CoA, and 2-methylhexanoyl-CoA), as well as toward the short straight chain acyl-CoAs (butyryl-CoA and hexanoyl-COA). (C) 1994 Academic Press, Inc.