GENERATION OF IC3 AT THE INTERFACE BETWEEN BLOOD AND GAS
GENERATION OF IC3 AT THE INTERFACE BETWEEN BLOOD AND GAS
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DOI：
10.1111/j.1365-3083.1992.tb02837.x
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发表时间：
1992-01-01
影响因子：
3.7
通讯作者：
NILSSON, UR
中科院分区：
文献类型：
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作者：
EKDAHL, KN;NILSSON, B;NILSSON, UR
先前的研究表明，当血液与聚苯乙烯表面接触时，C3会发生变性。本研究旨在探究在心肺手术中使用的各类氧合器中存在的气 - 血浆界面是否会发生类似的C3变性。采用了一个体外系统，即让气体鼓泡通过人血、血清或血浆。对作为补体激活指标的C3a的产生以及iC3和iC3片段进行了监测。鼓泡过程中，C3a以及iC3/iC3片段的水平均升高。与C3a水平不同的是，在乙二胺四乙酸（EDTA）存在的情况下，未观察到iC3/iC3片段形成减少，这表明它与补体激活无关。iC3/iC3片段的生成速率不受气体成分（纯氧、纯氮或空气）的影响，这表明C3的变性确实发生在血清 - 气体界面。通过聚乙二醇沉淀以及在快速蛋白液相色谱（FPLC）上使用Mono S柱进行色谱分析，从鼓泡的EDTA螯合血清中分离出C3和iC3/iC3片段，并使用两种分别针对天然C3和iC3/iC3片段的酶联免疫吸附测定（ELISA）进行检测。240分钟后，大约20%的C3总量由完整的iC3组成，并且证实这部分iC3可与人红细胞结合。
Earlier studies have shown that C3 can be denatured when blood comes in contact with a polystyrene surface. This study was undertaken to see if similar denaturation of C3 occurs at the gas-plasma interface which is found in all kinds of oxygenator used during cardio-pulmonary operations. An in vitro system consisting of gas bubbling through human blood, serum or plasma was used. The generation of C3a, as an indicator of complement activation, and iC3 and iC3 fragments were monitored. Both C3a and iC3/iC3 fragments levels were increased during bubbling. In contrast to the C3a level, no reduction in iC3/iC3 fragments formation was seen in the presence of EDTA, indicating that it was independent of complement activation. The rate of iC3/iC3 fragments generation was unaffected by the composition of the gas (pure oxygen, pure nitrogen or air), suggesting that the denaturation of C3 indeed occurred at the serum-gas interface. C3 and iC3/iC3 fragments were isolated from bubbled EDTA-chelated serum by PEG precipitation and chromatography on FPLC, using a Mono S column and detected by two ELISAs, specific for native C3 and iC3/iC3 fragments. After 240 min approximately 20% of the total amount of C3 consisted of intact iC3 and it was confirmed that this population bound to human erythrocytes.