ALPHA-ACTININ INTERACTS SPECIFICALLY WITH MODEL MEMBRANES CONTAINING GLYCERIDES AND FATTY-ACIDS
ALPHA-ACTININ INTERACTS SPECIFICALLY WITH MODEL MEMBRANES CONTAINING GLYCERIDES AND FATTY-ACIDS
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DOI：
10.1073/pnas.79.14.4280
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
BURGER, MM
中科院分区：
文献类型：
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作者：
MEYER, RK;SCHINDLER, H;BURGER, MM
开发了一种方法来识别复杂脂质混合物中特定的蛋白质-脂质相互作用，并评估它们对这种复合体在空气-水界面的单分子层中排列的影响。它对横纹肌α-肌动蛋白的应用表明，只有2种脂类与α-肌动蛋白选择性地相互作用。一个甘油三酯分子和一个脂肪酸分子以恒定的化学计量比与一个α-肌动蛋白二聚体分子相连。在甘油三酯和脂肪酸同时存在的情况下，出人意料地形成了刚性单层区域。这种脂类专一性可以通过对α-肌动蛋白脂质体混合物进行简单的蛋白酶消化然后进行多肽分析来证实；α-肌动蛋白的多肽模式取决于是否只有这两种脂类。在Z线形成的背景下，讨论了这些发现的可能的含义。
A method was developed to identify specific protein-lipid interactions of complex lipid mixtures and to assess their effect on the arrangement of such complexes in monolayers at an air-water interface. Its application to striated [pig] muscle .alpha.-actinin revealed that just 2 lipids selectively interact with .alpha.-actinin. One molecule of glyceride and 1 molecule of fatty acid were associated in a constant stoichiometry with 1 molecule of the .alpha.-actinin dimer. In the presence of both glycerides and fatty acids unexpectedly rigid monolayer areas formed. This lipid specificity could be confirmed by brief protease digestion of .alpha.-actinin liposome mixtures followed by peptide analysis; the peptide patterns of .alpha.-actinin depended on the presence or absence of only these 2 lipids. Possible implications of these findings are discussed in the context of Z-line formation.