Use of an aberrant polypeptide as a marker in three-factor crosses: further evidence for independent reassortment as the mechanism of recombination between temperature-sensitive mutants of reovirus type 3.
Use of an aberrant polypeptide as a marker in three-factor crosses: further evidence for independent reassortment as the mechanism of recombination between temperature-sensitive mutants of reovirus type 3.
复制标题
使用异常多肽作为三因子杂交中的标记：独立重配作为 3 型呼肠孤病毒温度敏感突变体之间重组机制的进一步证据。
DOI：
10.1016/0042-6822(76)90341-x
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发表时间：
1976
期刊：
影响因子：
3.7
通讯作者：
B. Fields
中科院分区：
文献类型：
--
作者：
R. Cross;B. Fields
The aberrant μ1 and μ2 viral polypeptide phenotype (designatedμ−−) of certain groups of temperature-sensitive mutants of reovirus type 3 has been utilized as a third unselected genetic marker in three-factor crosses. Approximately 50% of thets+recombinant clones were found to express the wild typeμ+phenotype of one of the parents, while the remainder expressed the aberrantμ−−phenotype of the other. The approximately random distribution of the μ phenotype in two crosses of this type supports the conclusion that recombinants which result from mixed infection with pairs of temperature-sensitive mutants of reovirus type 3 are generated by random assortment of RNA segments. The results of three-factor crosses, furthermore, suggest that thetsmutation of the group A mutant (ts201) is genetically linked to the determinant of the aberrant μ1 and μ2 polypeptide.In addition,ts+revertants of groups B, D, and G retain the μ phenotype of the mutant strain from which they are derived, while the group A (ts201) revertants display a variety of phenotypes.
