Th17 Cell Induction by Adhesion of Microbes to Intestinal Epithelial Cells.
Th17 Cell Induction by Adhesion of Microbes to Intestinal Epithelial Cells.
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DOI：
10.1016/j.cell.2015.08.058
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发表时间：
2015-10-08
期刊：
影响因子：
64.5
通讯作者：
Honda K
中科院分区：
文献类型：
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作者：
Atarashi K;Tanoue T;Ando M;Kamada N;Nagano Y;Narushima S;Suda W;Imaoka A;Setoyama H;Nagamori T;Ishikawa E;Shima T;Hara T;Kado S;Jinnohara T;Ohno H;Kondo T;Toyooka K;Watanabe E;Yokoyama S;Tokoro S;Mori H;Noguchi Y;Morita H;Ivanov II;Sugiyama T;Nuñez G;Camp JG;Hattori M;Umesaki Y;Honda K
肠道Th17细胞在肠道微生物亚群如节段性丝状细菌(SFB)和某些细胞外病原体的侵袭下被诱导和聚集。在这里，我们表明微生物对肠上皮细胞(ECs)的黏附是Th17诱导的关键信号。在小鼠(M-SFB)或大鼠(R-SFB)的无菌小鼠或带有SFB的大鼠(M-SFB)中，M-SFB和R-SFB表现出宿主特异性的黏附于小肠内皮细胞，并伴随宿主特异性的Th17细胞诱导。轮状柠檬酸杆菌和大肠杆菌O157引发了类似的Th17反应，而这些微生物的黏附缺陷突变体未能做到这一点。此外，从一名溃疡性结肠炎患者的粪便样本中挑选和分离的20株细菌的混合物也显示出EC黏附特征。这些细菌是根据它们在小鼠结肠中诱导Th17细胞的能力而分离出来的。
Intestinal Th17 cells are induced and accumulate in response to colonization with a subgroup of intestinal microbes such as segmented filamentous bacteria (SFB) and certain extracellular pathogens. Here, we show that adhesion of microbes to intestinal epithelial cells (ECs) is a critical cue for Th17 induction. Upon monocolonization of germ-free mice or rats with SFB indigenous to mice (M-SFB) or rats (R-SFB), M-SFB and R-SFB showed host-specific adhesion to small intestinal ECs, accompanied by host-specific induction of Th17 cells. Citrobacter rodentium and Escherichia coli O157 triggered similar Th17 responses, whereas adhesion-defective mutants of these microbes failed to do so. Moreover, a mixture of 20 bacterial strains, which were selected and isolated from fecal samples of a patient with ulcerative colitis on the basis of their ability to cause a robust induction of Th17 cells in the mouse colon, also exhibited EC-adhesive characteristics.