Hydrogen Sulfide and Hydrogen Sulfide-Synthesizing Enzymes Are Altered in a Case of Oral Adenoid Cystic Carcinoma.
Hydrogen Sulfide and Hydrogen Sulfide-Synthesizing Enzymes Are Altered in a Case of Oral Adenoid Cystic Carcinoma.
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DOI：
10.1159/000492464
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发表时间：
2018-05
影响因子：
0.8
通讯作者：
Shackelford RE
中科院分区：
文献类型：
--
作者：
Dongsoo K;Chen J;Wei E;Ansari J;Meram A;Patel S;Ghali G;Kevil C;Shackelford RE
腺样囊性癌（ACC）占所有头颈部恶性肿瘤的1%，在口腔中非常罕见。描述了< 60种口服ACC，其病理生物学不完全清楚。在这里，我们报告一个54岁的女性口腔ACC的情况。由于最近的研究表明，几种人类肿瘤过表达硫化氢（H2S）合成酶胱硫醚-β-合成酶（CBS），胱硫醚γ-裂解酶（CSE）和3-巯基丙酮酸硫转移酶（3-MST），也显示失调的H2S水平，我们检查了口腔ACC中的这些生物标志物，并将结果与邻近良性口腔上皮的结果进行了比较。Western blotting检测ACC及癌旁组织中CBS、CSE、3-MST、烟酰胺磷酸核糖转移酶和mitoNEET的蛋白表达。高效液相色谱法被用来量化两种活检类型之间的组织H2S浓度的差异。我们发现，与邻近的良性口腔粘膜相比，ACC中检测的所有蛋白质都增加了。有趣的是，与良性粘膜相比，ACC中的H2S浓度降低约30%。因此，在这种罕见肿瘤类型的一个例子中，合成H2S的酶增加，而组织H2S水平低于邻近良性口腔粘膜中发现的水平。虽然仅限于单一罕见肿瘤类型，但据我们所知，这是第二次直接定量人类肿瘤内的H2S浓度。最后，我们的研究结果可能表明，H2S合成和代谢的改变可能是重要的ACC的病理生物学。
Adenoid cystic carcinomas (ACC) constitute 1% of all head and neck malignancies and are very rare in the oral cavity. With < 60 oral ACCs described, their pathobiology is incompletely understood. Here, we report a case of oral cavity ACC in a 54-year-old woman. Since recent studies have demonstrated that several human tumors overexpress the hydrogen sulfide (H2S)-synthesizing enzymes cystathionine-β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (3-MST), and also show dysregulated H2S levels, we examined these biomarkers in the oral ACC and compared the results to those of adjacent benign oral epithelium. Western blotting was used to compare the protein expression of CBS, CSE, 3-MST, nicotinamide phosphoribosyl transferase, and mitoNEET in ACC and adjacent benign oral mucosae. High-performance liquid chromatography was used to quantify the differences in tissue H2S concentrations between the two biopsy types. We found that all the proteins examined here were increased in the ACC compared to adjacent benign oral mucosae. Interestingly, H2S concentrations were decreased approximately 30% in ACC compared to benign mucosae. Thus, in one example of this rare tumor type, the enzymes that synthesize H2S are increased, while tissue H2S levels are lower than those found in adjacent benign oral mucosae. Although limited to a single rare tumor type, to our knowledge this is the second time H2S concentrations have been directly quantified inside a human tumor. Last, our results may indicate that alterations in H2S synthesis and metabolism may be important in the pathobiology of ACC.