Fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enhancing enzymatic saccharification and hydrogen production.
Fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enhancing enzymatic saccharification and hydrogen production.
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DOI：
10.1016/j.biortech.2012.03.076
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发表时间：
2012-06
影响因子：
11.4
通讯作者：
Lei Zhao;Guang-li Cao;Ai-Jie Wang;Hongyu Ren;D. Dong;Zi-Nan Liu;Xiao-Yu Guan;Chengjiao Xu;N. Ren
中科院分区：
文献类型：
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作者：
Lei Zhao;Guang-li Cao;Ai-Jie Wang;Hongyu Ren;D. Dong;Zi-Nan Liu;Xiao-Yu Guan;Chengjiao Xu;N. Ren
本研究探讨了用黄孢原毛平革菌对玉米秆进行酶促糖化产氢的可行性。首先，用黄孢青霉在29℃静态条件下预处理玉米秸秆15d，木质素减少达34.3%，全纤维素损失小于10%。微观结构观察结合FTIR分析进一步表明木质素和结晶度降低。随后，用来自绿色木霉的粗纤维素酶对经过真菌预处理的玉米杆进行酶水解，产生可发酵糖，然后使用热解糖厌氧杆菌W16将其发酵为生物H2。最大酶解糖化率为47.3%，比未经预处理的对照高20.3%。酶解产物发酵后，经计算，H2 产量为 80.3ml/g（预处理的玉米杆）。目前的结果表明，结合生物预处理和酶糖化，利用产氢细菌将玉米秆高产转化为生物氢的潜力。
The feasibility of fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enzymatic saccharification and H2production was investigated in this study. Firstly, cornstalk was pretreated with P. chrysosporium at 29°C under static condition for 15d, lignin reduction was up to 34.3% with holocellulose loss less than 10%. Microscopic structure observation combined FTIR analysis further demonstrated that the lignin and crystallinity were decreased. Subsequently, the fungal-pretreated cornstalk was subjected to enzymatic hydrolysis by the crude cellulase from Trichoderma viride to produce fermentable sugars which were then fermented to bio-H2using Thermoanaerobacterium thermosaccharolyticum W16. The maximum enzymatic saccharification was found to be 47.3% which was 20.3% higher than the control without pretreatment. Upon fermentation of enzymatic hydrolysate, the yield of H2was calculated to be 80.3ml/g-pretreated cornstalk. The present results suggested the potential of using hydrogen-producing bacteria for high-yield conversion of cornstalk into bio-H2integrate with biological pretreatment and enzymatic saccharification.