Dynamic recruitment of amino acid transporters to the insect/symbiont interface
Dynamic recruitment of amino acid transporters to the insect/symbiont interface
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DOI：
10.1111/mec.12627
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发表时间：
2014-03-01
影响因子：
4.9
通讯作者：
Wilson, Alex C. C.
中科院分区：
文献类型：
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作者：
Duncan, Rebecca P.;Husnik, Filip;Wilson, Alex C. C.
众所周知，共生关系会影响细菌共生体的基因组进化，最近也有研究表明，共生关系会影响真核宿主的基因组。寄主基因组进化的有趣模式，包括显著数量的基因复制，已经在豌豆蚜虫中观察到，豌豆蚜虫是一种依靠细菌内共生体提供氨基酸的取食昆虫。在此之前，我们提出了基于氨基酸转运体（AATs）中蚜虫特异性基因复制的共生进化中基因复制是重要的，一些类似的氨基酸转运体在共生细胞（细菌细胞）中高度表达。在这里，我们使用比较方法来测试基因复制在使aat招募到细菌细胞中的作用。利用基因组和转录组学数据，我们对取食汁液和不取食汁液的昆虫的AAT基因进行了注释，发现与蚜虫一样，AAT基因家族在另外四种取食汁液的昆虫中有三种经历了独立的大规模基因复制。RNA-seq差异表达数据表明，像蚜虫一样，取食柑橘粉蚧具有几个谱系特异性的富含细菌细胞的类似物。此外，结合定量PCR的差异表达数据支持取食昆虫AATs中细菌细胞富集的独立进化。虽然这些数据表明基因复制不是启动宿主/共生体氨基酸交换的必要条件，但它们支持基因复制在使aat广泛介导寄主/共生体相互作用中的作用。结合最近对其他共生系统的研究，基因复制正在成为宿主基因组进化的一种普遍模式。
Symbiosis is well known to influence bacterial symbiont genome evolution and has recently been shown to shape eukaryotic host genomes. Intriguing patterns of host genome evolution, including remarkable numbers of gene duplications, have been observed in the pea aphid, a sap-feeding insect that relies on a bacterial endosymbiont for amino acid provisioning. Previously, we proposed that gene duplication has been important for the evolution of symbiosis based on aphid-specific gene duplication in amino acid transporters (AATs), with some paralogs highly expressed in the cells housing symbionts (bacteriocytes). Here, we use a comparative approach to test the role of gene duplication in enabling recruitment of AATs to bacteriocytes. Using genomic and transcriptomic data, we annotate AATs from sap-feeding and non sap-feeding insects and find that, like aphids, AAT gene families have undergone independent large-scale gene duplications in three of four additional sap-feeding insects. RNA-seq differential expression data indicate that, like aphids, the sap-feeding citrus mealybug possesses several lineage-specific bacteriocyte-enriched paralogs. Further, differential expression data combined with quantitative PCR support independent evolution of bacteriocyte enrichment in sap-feeding insect AATs. Although these data indicate that gene duplication is not necessary to initiate host/symbiont amino acid exchange, they support a role for gene duplication in enabling AATs to mediate novel host/symbiont interactions broadly in the sap-feeding suborder Sternorrhyncha. In combination with recent studies on other symbiotic systems, gene duplication is emerging as a general pattern in host genome evolution.