DERMAL GRANULOMATOUS HYPERSENSITIVITY IN Q-FEVER - COMPARATIVE STUDIES OF THE GRANULOMATOUS POTENTIAL OF WHOLE CELLS OF COXIELLA-BURNETII PHASE-I AND SUBFRACTIONS
DERMAL GRANULOMATOUS HYPERSENSITIVITY IN Q-FEVER - COMPARATIVE STUDIES OF THE GRANULOMATOUS POTENTIAL OF WHOLE CELLS OF COXIELLA-BURNETII PHASE-I AND SUBFRACTIONS
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DOI：
10.1128/iai.42.3.887-889.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
BERMAN, MA
中科院分区：
文献类型：
--
作者：
ASCHER, MS;WILLIAMS, JC;BERMAN, MA
利用豚鼠皮肤肉芽肿对Q热抗原的反应性，研究了伯氏梭菌亚组分产生肉芽肿的能力。对Q热菌I期、三氯乙酸组分、三氯乙酸不解组分、三氯甲烷甲醇提取物和残留物对豚鼠皮肤肉芽肿反应和特异性淋巴细胞增殖反应的相对诱导和免疫能力进行了检测。显然，引起肉芽肿的决定簇(S)可以通过I相全细胞的CHCl3-甲醇提取来去除。CHCl3-甲醇残留物引起强烈的迟发性超敏反应，没有随后的肉芽肿形成。三氯甲烷-甲醇残留物似乎具有一个与整个I相生物体相当的淋巴细胞刺激决定子(S)。
Dermal granulomatous reactivity to Q fever antigens in guinea pigs was applied to study the ability of subfractions of C. burnetii to produce granulomas. Q fever organisms in phase I, trichloroacetic acid-soluble and -insoluble fractions and the extract and residue of CHCl3 methanol extraction were tested for their relative ability to elicit and immunize for dermal granulomatous reactions and specific lymphocyte proliferative responses in guinea pigs. Evidently, a determinant(s) causing granulomas can be removed by CHCl3-methanol extraction of phase I whole cells. The CHCl3-methanol residue elicited strong delayed-type hypersensitivity without subsequent granuloma formation. The CHCL3-methanol residue appears to possess a determinant(s) for lymphocyte stimulation equivalent to that of whole phase I organisms.