DNA replication-dependent formation of joint DNA molecules in Physarum polycephalum
DNA replication-dependent formation of joint DNA molecules in Physarum polycephalum
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DOI：
10.1016/s1097-2765(01)00237-4
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发表时间：
2001-05-01
期刊：
影响因子：
16
通讯作者：
Pierron, G
中科院分区：
文献类型：
--
作者：
Bénard, M;Maric, C;Pierron, G
二维中性/中性琼脂糖凝胶电泳广泛用于定位复制起点。这种方法可以解析包含复制叉的DNA结构。它还可以检测减数分裂细胞中的x形重组中间体，以典型的垂直突起的形式。有趣的是，在有丝分裂细胞的DNA复制过程中，经常可以检测到这种联合DNA分子的尖峰。在这里，我们使用来自多头绒泡菌的自然同步DNA样本来证明复制后，DNA复制依赖的x形DNA分子在姐妹染色单体之间形成。这些分子的物理性质让人想起假日结。我们的研究结果表明在正常细胞周期中姐妹染色单体之间的频繁相互作用，并表明在DNA复制过程中存在一个新的阶段，该阶段由在新复制的DNA上形成的瞬时联合DNA分子组成。
Two-dimensional neutral/neutral agarose gel electrophoresis is used extensively to localize replication origins. This method resolves DNA structures containing replication forks. It also detects X-shaped recombination intermediates in meiotic cells, in the form of a typical vertical spike. Intriguingly, such a spike of joint DNA molecules is often detectable in replicating DNA from mitotic cells. Here, we used naturally synchronous DNA samples from Physarum polycephalum to demonstrate that postreplicative, DNA replication-dependent X-shaped DNA molecules are formed between sister chromatids. These molecules have physical properties reminiscent of Holliday junctions. Our results demonstrate frequent interactions between sister chromatids during a normal cell cycle and suggest a novel phase during DNA replication consisting of transient, joint DNA molecules formed on newly replicated DNA.