Bamboo leaf flavone changed the community of cecum microbiota and improved the immune function in broilers
Bamboo leaf flavone changed the community of cecum microbiota and improved the immune function in broilers
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竹叶黄酮改变盲肠微生物群落，提高肉鸡免疫功能
DOI：
10.1038/s41598-020-69010-1
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发表时间：
2020-07-23
影响因子：
4.6
通讯作者：
Zhao, Xiaoling
中科院分区：
文献类型：
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作者：
Shu, Gang;Kong, Fanli;Zhao, Xiaoling
竹叶黄酮（BLF）在哺乳动物体内具有生物学和药理学活性。然而，BLF对肉鸡肠道菌群和相关免疫功能的影响尚未研究。本研究的目的是验证我们的假设，即BLF可以通过调节肠道微生物群来改善肉鸡的健康状况。试验选用300只1日龄AA肉仔鸡，在日粮中添加BLF，研究其肠道菌群和免疫功能的变化。通过Illumina MiSeq平台对来自盲肠细菌的16 S rRNA基因的V4高变区进行测序。检测免疫器官指数（脾脏、胸腺和法氏囊）、血清IL-2和INF-γ浓度以及脾脏IL-2和INF-γ基因表达。结果表明，BLF日粮对肉仔鸡具有免疫增强作用。此外，BFL还引起肠道微生物群落结构的变化，导致乳酸杆菌、梭菌目、瘤胃球菌和毛螺菌科的细菌类群比例增加。这些细菌已被用作在宿主中产生短链脂肪酸的益生菌。这些结果表明，BLF补充剂通过调节肠道微生物群来改善鸡的免疫功能。
It has been shown that bamboo leaf flavone (BLF) displays biological and pharmacological activities in mammals. However, the effects of BLF on broiler gut microbiota and related immune function have not been investigated. The aim of this study was to test our hypothesis that BLF can improve the health status of broilers by modulating the gut microbiota. A total of 300 one-day-old Arbor Acres (AA) broilers were used to characterize their gut microbiota and immune status after feeding diet supplemented with BLF. The V4 hypervariable region of the 16S rRNA gene from cecal bacteria was sequenced via the Illumina MiSeq platform. The Immune status and related parameters were assessed, including the immune organ index (the spleen, thymus, and bursa), serum concentrations of IL-2 and INF-γ, and spleenIL-2andINF-γgene expressions. The results showed the BLF diet had an Immune enhancement effect on broilers. In addition, BFL caused the changes of the gut microbial community structure, resulting in greater proportions of bacterial taxa belonging toLactobacillus,Clostridiales,Ruminococcus, andLachnospiraceae. These bacteria have been used as probiotics for producing short chain fatty acids in hosts. These results indicate that BLF supplement improves immune function in chicken via modulation of the gut microbiota.