The Arabidopsis eIF4E1 regulates NRT1.1-mediated nitrate signaling at both translational and transcriptional levels
The Arabidopsis eIF4E1 regulates NRT1.1-mediated nitrate signaling at both translational and transcriptional levels
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DOI：
10.1111/nph.19129
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发表时间：
2023-07-10
期刊：
影响因子：
9.4
通讯作者：
Wang，Yong
中科院分区：
文献类型：
--
作者：
Li，Na;Duan，Yawen;Wang，Yong
识别新的硝酸盐调控基因并阐明其调控硝酸盐信号的机制对于实现作物高产和氮素利用效率（NUE）具有重要意义。在这里，我们筛选了一个突变体与缺陷的硝酸盐反应和定位突变的基因eIF 4 E1在拟南芥。我们的研究结果表明，eIF 4 E1调节硝酸盐信号和代谢。Ribo-seq和多核糖体分析表明，eIF 4 E1调节了一些氮（N）相关mRNA的翻译量，特别是NRT 1的mRNA。1在eif 4 e1突变体中减少。RNA-Seq结果丰富了一些N相关基因，支持eIF 4 E1参与硝酸盐调控。遗传分析表明eIF 4 E1在NRT 1的上游起作用。1在硝酸盐信号中。此外，还鉴定了一种eIF 4 E1相互作用蛋白GEMIN 2，并发现其参与硝酸盐信号传导。进一步的研究表明eIF 4 E1的过表达促进了植株的生长，提高了产量和氮利用效率。这些结果表明eIF 4 E1通过调节NRT 1来调节硝酸盐信号传导。1在翻译和转录水平上的表达，为今后在翻译水平上研究矿物质营养的调控奠定了基础。
Identifying new nitrate regulatory genes and illustrating their mechanisms in modulating nitrate signaling are of great significance for achieving the high yield and nitrogen use efficiency (NUE) of crops. Here, we screened a mutant with defects in nitrate response and mapped the mutation to the gene eIF4E1 in Arabidopsis. Our results showed that eIF4E1 regulated nitrate signaling and metabolism. Ribo‐seq and polysome profiling analysis revealed that eIF4E1 modulated the amount of some nitrogen (N)‐related mRNAs being translated, especially the mRNA of NRT1. 1 was reduced in the eif4e1 mutant. RNA‐Seq results enriched some N‐related genes, supporting that eIF4E1 is involved in nitrate regulation. The genetic analysis indicated that eIF4E1 worked upstream of NRT1. 1 in nitrate signaling. In addition, an eIF4E1‐interacting protein GEMIN2 was identified and found to be involved in nitrate signaling. Further investigation showed that overexpression of eIF4E1 promoted plant growth and enhanced yield and NUE. These results demonstrate that eIF4E1 regulates nitrate signaling by modulating NRT1. 1 at both translational and transcriptional levels, laying the foundation for future research on the regulation of mineral nutrition at the translational level.