Characterization of methylthioadenosin phosphorylase (MTAP) expression in colorectal cancer
Characterization of methylthioadenosin phosphorylase (MTAP) expression in colorectal cancer
复制标题
结直肠癌中甲硫腺苷磷酸化酶 (MTAP) 表达的特征
DOI：
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发表时间：
2017
影响因子：
5.8
通讯作者：
Shanming Sun
中科院分区：
文献类型：
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作者：
Yanmei Zhong;Keliang Lu;Suhua Zhu;Wentong Li;Shanming Sun
摘要目的：肿瘤严重影响人们的生活质量。结直肠癌是消化道肿瘤中的一种难治性肿瘤。在结直肠癌中，基因表达异常是其发生的主要原因，我们主要关注MTAP在结直肠癌发生发展中的分子机制。方法：对2011年7月至2015年2月收治的50例结直肠癌患者的肿瘤组织及癌旁组织标本进行筛查，并检测MTAP的表达。建立了高表达MTAP和低表达MTAP的结直肠癌细胞株。检测不同MTAP表达水平的细胞增殖、侵袭和迁移情况。应用免疫组织化学方法检测MTAP在肝转移瘤中的表达，并探讨其临床意义。具有临床意义的统计数据。结果：Q-PCR结果显示，MTAP在结直肠癌细胞系中的表达明显高于正常人结肠肌成纤维细胞系。细胞增殖实验结果显示，当MTAP过表达时，细胞增殖加快，细胞侵袭和迁移同时加速。肝转移结直肠癌患者原发肝组织中MTAP的表达与肝转移呈正相关。结论：MTAP通过EMT促进结直肠癌的生长和转移。
Abstract Purpose: Tumour seriously affects people's quality of life. Colorectal cancer is a refractory tumour in digestive tract tumors. In colorectal cancer, gene expression abnormalities is the main reason for its incidence, we mainly focus on the molecular mechanism of MTAP in the development of colorectal cancer. Methods: The tumour tissue and its adjacent tissue samples of 50 patients with colorectal cancer were screened from July 2011 to February 2015, and the expression of MTAP was detected. Cell lines that overexpress MTAP and low expression of MTAP were constructed in colorectal cancer cell lines. The cell proliferation, invasion and migration was detected in the cells with different expression levels of MTAP. Immunohistochemistry was used to detect the expression of MTAP in liver metastasis and to investigate its clinical significance. And statistics of clinical significance. Results: Q-PCR results showed that the expression of MTAP in colorectal cancer cell lines were significantly higher than that normal human colonic myofibroblasts cell line. Cell proliferation test results showed that cell proliferation was accelerated when MTAP was overexpression, cell invasion and migration were simultaneously accelerated. The expression of MTAP in primary liver was positively correlated with metastatic disease in patients with liver metastatic colorectal cancer via EMT. Conclusions: MTAP accelerates the growth and metastasis of colorectal cancer through EMT.