Distribution of Pink-Pigmented Facultative Methylotrophs on Leaves of Vegetables
Distribution of Pink-Pigmented Facultative Methylotrophs on Leaves of Vegetables
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DOI：
10.1271/bbb.110737
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发表时间：
2012-03
期刊：
影响因子：
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通讯作者：
M. Mizuno;H. Yurimoto;N. Yoshida;H. Iguchi;Y. Sakai
中科院分区：
文献类型：
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作者：
M. Mizuno;H. Yurimoto;N. Yoshida;H. Iguchi;Y. Sakai
研究了粉红色兼性甲基营养菌（PPFM）在各种蔬菜叶子上的分布。所测试的各种蔬菜叶子在含有甲醇作为唯一碳源的琼脂平板上产生粉红色的菌落。尽管它们是在同一农场种植和生长的，但叶子上的 PPFM 数量（菌落形成单位（CFU）/克新鲜叶子）在不同植物之间有所不同。在所有测试的商业蔬菜叶子中，商业绿紫苏 Perilla frutescens viridis (Makino) Makino 的 PPFM 计数最高（2.0–4.1×107 CFU/g），占叶子上微生物总数的 15%。从两个紫苏品种（红色和绿色品种）的种子中分离的 PPFM，在 16S rRNA 基因方面与两个不同的甲基杆菌物种（分别是 M. fujisawaense DSM5686T 和 M. radiotolerans JCM2831T）表现出高度的序列相似性，表明紫苏和 PPFM 之间存在特定的相互作用。
The distribution of pink-pigmented facultative methylotrophs (PPFMs) on the leaves of various vegetables was studied. All kinds of vegetable leaves tested gave pink-pigmented colonies on agar plates containing methanol as sole carbon source. The numbers of PPFMs on the leaves, colony-forming units (CFU)/g of fresh leaves, differed among the plants, although they were planted and grown at the same farm. Commercial green perilla, Perilla frutescens viridis (Makino) Makino, gave the highest counts of PPFMs (2.0–4.1×107 CFU/g) of all the commercial vegetable leaves tested, amounting to 15% of total microbes on the leaves. The PPFMs isolated from seeds of two varieties of perilla, the red and green varieties, exhibited high sequence similarity as to the 16S rRNA gene to two different Methylobacterium species, M. fujisawaense DSM5686T and M. radiotolerans JCM2831T respectively, suggesting that there is specific interaction between perilla and the PPFMs.