GENETIC-ANALYSIS OF BACILLUS-STEAROTHERMOPHILUS BY PROTOPLAST FUSION
GENETIC-ANALYSIS OF BACILLUS-STEAROTHERMOPHILUS BY PROTOPLAST FUSION
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DOI：
10.1128/jb.165.3.994-1001.1986
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发表时间：
1986-03-01
影响因子：
3.2
通讯作者：
WELKER, NE
中科院分区：
文献类型：
--
作者：
CHEN, Z;WOJCIK, SF;WELKER, NE
为原养脂肪嗜热芽孢杆菌NUB36建立了高效、可靠的原生质体、再生和融合技术。分离出营养缺陷型突变株，利用原生质体融合技术构建等基因突变株并进行染色体定位。标记用两因素、三因素和四因素杂交作图。标记顺序为HOM-1-THR-1-His-1-(Gly-1或Gly-2-)-Pur-1-Pur-2。这些标记可能类似于枯草芽孢杆菌染色体上的hom、thra、hisa、lyc和Pura标记。在枯草杆菌中还没有类似的pur-1分子的报道。其中三个标记(hom-1-thr-1-Gly-1)的相对顺序通过转导被独立地证实。
Efficient and reliable protoplasting, regeneration, and fusion techniques were established for the prototrophic strain Bacillus stearothermophilus NUB36. Auxotrophic mutants were isolated, and protoplast fusion was used to construct isogenic mutant strains and for chromosomal mapping. Markers were mapped using two-, three-, and four-factor crosses. The order of the markers was hom-1-thr-1-his-1-(gly-1 or gly-2-)-pur-1-pur-2. These markers may be analogous to hom, thrA, hisA, glyC, and purA markers on the Bacillus subtilis chromosome. No analogous pur-1 maker has been reported in B. subtilis. The relative order of three of the markers (hom-1-thr-1-gly-1) was independently confirmed by transduction.