Cell-type-dependent induction of eotaxin and CCR3 by ionizing radiation
Cell-type-dependent induction of eotaxin and CCR3 by ionizing radiation
复制标题
DOI：
10.1006/bbrc.2000.2287
复制
发表时间：
2000-03-16
影响因子：
3.1
通讯作者：
Peter, RU
中科院分区：
文献类型：
--
作者：
Huber, MA;Kraut, N;Peter, RU
嗜酸性粒细胞趋化因子是一种嗜酸性粒细胞特异性C-C趋化因子，通过特异性作用于其受体CCR3，参与嗜酸性炎症性疾病，如哮喘和特应性皮炎的发病机制。利用RT-PCR分析，我们发现电离辐射(Ht)处理的人真皮成纤维细胞中嗜酸粒细胞趋化因子的表达上调，但在支气管上皮细胞系A549中不表达。相反，编码CCR3的基因在这两种细胞中都被显著诱导。在这些类型的细胞中，Ut没有显著诱导编码其他CCR3配体的基因。对照射与未照射的A549细胞和人真皮成纤维细胞的cDNA阵列分析证实并扩展了这些结果，并支持Ut以选择性和细胞类型依赖的方式调节eoaxin/CCR3-诱导的观察。他们进一步表明，通过嗜酸性粒细胞趋化蛋白和CCR3诱导信号可能是导致辐射患者嗜酸性粒细胞增多症的重要步骤。(C)2000年学术出版社。
Eotaxin is an eosinophil-specific C-C chemokine that is implicated in the pathogenesis of eosinophilic inflammatory diseases, such as asthma and atopic dermatitis, by acting specifically on its receptor CCR3. Using RT-PCR analysis, we show that the expression of eotaxin is upregulated upon treatment with ionizing radiation (Ht) in human dermal fibroblasts, but not in the bronchial epithelial cell line A549. In contrast, the gene encoding CCR3 is markedly induced in both cell types. None of the genes coding for other CCR3 ligands are significantly induced by Ut in these cell types. cDNA array analysis of irradiated versus nonirradiated A549 cells and human dermal fibroblasts confirm and extend these results, and support the observation that regulation of eotaxin/CCR3-induction by Ut occurs in a selective and cell-type-dependent manner. They further suggest that the induction of signaling via eotaxin and CCR3 may be an important step leading to eosinophilia in patients with radiation exposure. (C) 2000 Academic Press.