In vitro growth rate of placental fibroblasts is developmentally regulated.
In vitro growth rate of placental fibroblasts is developmentally regulated.
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胎盘成纤维细胞的体外生长速率受发育调节。
DOI：
10.1172/jci115486
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Fant,ME
中科院分区：
文献类型：
--
作者：
Fant,ME
间充质来源的胎盘细胞用于在细胞水平上研究胎儿生长的调节。观察到胎盘成纤维细胞的体外生长率与胎龄的函数存在显着差异。源自 10-19 周胎盘的细胞的增殖率比源自 7-9 周胎盘的细胞高出两到三倍（16-30 小时与 30-60 小时，P 小于 0.001）。在培养物的多次传代中，增殖率保持稳定。此外，这两组细胞株对促有丝分裂刺激的反应表现出显着差异。使用最大有效浓度，胰岛素样生长因子 I 与表皮生长因子和成纤维细胞生长因子协同相互作用，刺激 10-19 周胎盘细胞中的 DNA 合成。相比之下，胰岛素样生长因子 1 与表皮生长因子和成纤维细胞生长因子的相互作用在 7-9 周的细胞中表现出明显较低的协同作用。这些发现表明，人类胎儿细胞的生长速度加快主要是由于细胞固有的发育事件造成的，并且与肽生长因子促有丝分裂作用的反应性增强有关。
Placental cells of mesenchymal origin were used to study the regulation of fetal growth at the cellular level. A significant difference in the in vitro growth rates of placental fibroblasts was observed as a function of gestational age. Cells derived from 10-19-wk placentae exhibited proliferative rates two to three times greater than cells derived from 7-9-wk placentae (16-30 h vs. 30-60 h, P less than 0.001). The proliferation rate remained stable throughout multiple passages in culture. Additionally, these two groups of cell strains exhibited marked differences in their responsiveness to mitogenic stimuli. Using maximal effective concentrations, insulin-like growth factor I interacted synergistically with epidermal growth factor and fibroblast growth factor to stimulate DNA synthesis in cells derived from 10-19-wk placentae. By contrast, the interaction of insulin-like growth factor 1 with epidermal growth factor and fibroblast growth factor exhibited significantly less synergy in 7-9-wk cells. These findings argue that the accelerated growth rate of human fetal cells results primarily from developmental events intrinsic to the cells and is associated with enhanced responsiveness to the mitogenic action of peptide growth factors.Images