Curvature-mediated cooperative wrapping of multiple nanoparticles at the same and opposite membrane sides
Curvature-mediated cooperative wrapping of multiple nanoparticles at the same and opposite membrane sides
复制标题
曲率介导的多个纳米粒子在相同和相对膜侧的协同包裹
DOI：
10.1039/c9nr03554k
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发表时间：
2019
期刊：
影响因子：
6.7
通讯作者：
Yue Tongtao
中科院分区：
文献类型：
--
作者：
Yan Zengshuai;Wu Zeming;Li Shixin;Zhang Xianren;Yi Xin;Yue Tongtao
细胞膜与纳米颗粒（NP）的相互作用对细胞功能至关重要，并且主要伴随着膜曲率的产生和传感。从膜的一侧诱导曲率的多个NP被认为通过曲率介导的相互作用被膜协同地包裹。然而，对另一种生物学上普遍存在的重要情况知之甚少，即，结合到相对膜侧的NP诱导不同方向的弯曲弯曲。结合粗粒度的分子动力学和理论分析，在这里，我们系统地研究了在相同和相对的膜两侧的多个粘合剂NP的包裹的合作效应，并证明了调节曲率介导的NP相互作用的膜弯曲的大小和方向的重要性。分析了纳米粒子的尺寸、尺寸差、初始距离、数量以及与膜的粘附强度对包裹协同性和包裹状态的影响。对于结合到同一膜侧的NP，观察到丰富的膜包裹和NP聚集状态，并且曲率介导的相互作用可以是吸引的或排斥的，这取决于初始NP距离以及膜弯曲、NP结合和膜突出之间的竞争。与此形成鲜明对比的是，结合到相对膜侧的两个NP之间的相互作用总是有吸引力的，并且促进了NP的合作包裹，因为由NP引起的弯曲膜区域以NP-膜接触增加并且膜弯曲的能量成本降低的方式被共享。由于膜形成蛋白在生物学中的普遍性和异质性，我们的研究结果丰富了曲率介导的纳米粒子相互作用的前沿知识，以更好地理解许多生物过程中纳米粒子或蛋白质的高阶合作组装。
Cell membrane interactions with nanoparticles (NPs) are essential to cellular functioning and mostly accompanied by membrane curvature generation and sensing. Multiple NPs inducing curvature from one side of a membrane are believed to be wrapped cooperatively by the membrane through curvature-mediated interactions. However, little is known about another biologically ubiquitous and important case, i.e., NPs binding to opposite membrane sides induce a curved bend of different directions. Combining coarse-grained molecular dynamics and theoretical analysis, here we systematically investigate the cooperative effect in the wrapping of multiple adhesive NPs at the same and opposite membrane sides and demonstrate the importance of the magnitude and direction of the membrane bend in regulating curvature-mediated NP interactions. Effects of the NP size, size difference, initial distance, number, and strength of adhesion with the membrane on the wrapping cooperativity and wrapping states are analyzed. For NPs binding to the same membrane side, rich membrane wrapping and NP aggregation states are observed, and the curvature-mediated interactions could be either attractive or repulsive, depending on the initial NP distance and the competition between the membrane bending, NP binding and membrane protrusion. In sharp contrast, the interaction between two NPs binding to opposite membrane sides is always attractive and the cooperative wrapping of NPs is promoted, as the curved membrane regions induced by the NPs are shared in a manner that the NP–membrane contact is increased and the energy cost of membrane bending is reduced. Owing to the ubiquity and heterogeneity of membrane shaping proteins in biology, our results enrich the cutting-edge knowledge on the curvature-mediated interaction of NPs for better and profound understanding on high-order cooperative assemblies of NPs or proteins in numerous biological processes.