Immunomodulation and antitumor activities of different-molecular-weight polysaccharides from Porphyridium cruentum
Immunomodulation and antitumor activities of different-molecular-weight polysaccharides from Porphyridium cruentum
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DOI：
10.1016/j.carbpol.2011.08.097
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发表时间：
2012-01-15
影响因子：
11.2
通讯作者：
Zhou, Yan
中科院分区：
文献类型：
--
作者：
Sun, Liqin;Wang, Ling;Zhou, Yan
采用超声波和密闭微波联合H2O2对芫荽叶卟啉的胞外多糖进行了降解研究。得到6个分子量分别为6.53、256、606、802.6、903.3和1002 kDa的产物。通过体内s180荷瘤小鼠模型和体外腹腔巨噬细胞活化实验，评价不同分子量多糖的抗肿瘤和免疫调节活性。降解的eps均表现出不同程度的明显免疫调节作用。eps的分子量对其活性有显著影响。其中6.53 kda片段的免疫增强活性最强。不同剂量EPS均能抑制S180肿瘤的生长。高、中、低剂量组肿瘤抑制指数分别为53.3%、47.5%、40.5%。此外，三种不同浓度EPS均能显著促进淋巴细胞增殖，提示EPS抗肿瘤作用的独特机制。(C) 2011 Elsevier Ltd.版权所有。
The extracellular polysaccharides (EPSs) isolated from Porphyridium cruentum were degraded by hermetical-microwave and H2O2 under ultrasonic waves. Six products were obtained with molecular weights of 6.53, 256, 606, 802.6, 903.3 and 1002 kDa. The antitumor and immunomodulatory activities of different-molecular-weight (MW) polysaccharides were evaluated by the S180-tumor-bearing mouse model in vivo and peritoneal macrophage activation in vitro. The degraded EPSs all showed clear immunomodulation to different extents. The MW of the EPSs had a notable effect on their activity. The 6.53-kDa fragment had the strongest immunoenhancing activity. Different doses of EPS all inhibited the growth of the implanted S180 tumor. The tumor inhibition index at high, middle and low doses was 53.3%, 47.5% and 40.5%, respectively. In addition, three different concentrations of EPS significantly increased lymphocyte proliferation, which indicated the unique mechanism of the antitumor effect of EPS. (C) 2011 Elsevier Ltd. All rights reserved.