Correlation between eicosanoids in bronchoalveolar lavage fluid and in exhaled breath condensate
Correlation between eicosanoids in bronchoalveolar lavage fluid and in exhaled breath condensate
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DOI：
10.1155/2011/323794
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发表时间：
2011-01-01
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影响因子：
--
通讯作者：
Barnes, Peter J.
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作者：
Antczak, Adam;Piotrowski, Wojciech;Barnes, Peter J.
呼出气冷凝物（EBC）已越来越多地用作研究气道炎症的新的非侵入性方法。在这项研究中，我们比较了 EBC 中脂质介质的浓度与支气管肺泡灌洗液 (BALF) 中的浓度。我们纳入了 37 名接受支气管镜检查的患者（12 例结节病、12 例慢性阻塞性肺病、6 例肺癌、5 例慢性咳嗽、1 例韦格纳肉芽肿病、1 例硬皮病）。在研究前至少 4 周内，患者不得出现病情加重或任何伴随药物变化。所有患者均在支气管镜检查前收集 EBC。BALF 中 cys-LTs、LTB4、8-异前列腺素的水平显着高于 EBC（cys-LTs、LTB4、8-异前列腺素分别为 p < 0.0001、p < 0.001、p < 0.0001）。此外，结节病和 COPD 患者的 BALF 和 EBC 中的白三烯 B-4 和 8-异前列腺素之间存在很强的正相关性（分别为 r = 0.53 和 r = 0.79，p < 0.01），但慢性咳嗽和肺癌患者的类花生酸 BALF 和 EBC 之间没有相关性。这是第一项比较不同肺部疾病中的 EBC 和 BALF 的研究，结果表明， COPD 和结节病患者 BALF 和 EBC 中类二十烷酸的水平。 EBC 可用于测量多种炎症性肺部疾病的炎症。
Exhaled breath condensate (EBC) has been increasingly used as a new and non-invasive method to study airway inflammation. In this study we have compared the concentrations of lipid mediators in EBC with concentrations in bronchoalveolar lavage fluid (BALF).We included 37 patients undergoing bronchoscopy (12 sarcoidosis, 12 COPD, 6 lung cancer, 5 chronic cough, 1 Wegener's granulomatosis, 1 sclerodermia). Patients were not allowed to have exacerbation or any change in concomitant medication for at least 4 weeks prior to the study. In all patients, EBC was collected immediately prior to the bronchoscopy.The levels of cys-LTs, LTB4, 8-isoprostane were significantly higher in BALF compared to EBC (p < 0.0001, p < 0.001, p < 0.0001 for cys-LTs, LTB4, 8-isoprostane respectively). Moreover, there was a strong positive correlation between both leukotriene B-4 and 8-isoprostane in BALF and EBC (r = 0.53 and r = 0.79, p < 0.01, respectively) in patients with sarcoidosis and COPD but there was no correlation between eicosanoids BALF and EBC in patients with chronic cough and lung cancer.This is the first study to compare EBC and BALF in different lung diseases which demonstrated significant correlations between the levels of eicosanoids in BALF and EBC in patients with COPD and sarcoidosis. EBC may be useful inmeasuring inflammation in several inflammatory lung diseases.