CHARACTERIZATION OF EPIDERMAL GLYCOSAMINOGLYCANS SYNTHESIZED IN ORGAN-CULTURE
CHARACTERIZATION OF EPIDERMAL GLYCOSAMINOGLYCANS SYNTHESIZED IN ORGAN-CULTURE
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DOI：
10.1016/0304-4165(81)90350-0
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
KING, IA
中科院分区：
文献类型：
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作者：
KING, IA
醋酸纤维素电泳和酶法及化学降解实验表明，在D-[3 H]氨基葡萄糖和35 SO_（42-）存在下培养的猪耳皮肤切片，表皮合成的糖胺聚糖主要是透明质酸（83%）和硫酸乙酰肝素（14%），胞外糖胺聚糖分别占总糖胺聚糖的81%和50%。培养5天后，总表皮糖胺聚糖合成减少了50%。当表皮在没有真皮的情况下培养时，透明质酸的合成大大减少。硫酸化糖胺聚糖的合成基本上不受真皮缺失的影响。全反式视黄酸10-5 M刺激透明质酸的合成，并在较小程度上硫酸化糖胺聚糖，无论是真皮缺席或存在于培养过程中。透明质酸可能在表皮分化的某些方面起重要作用。
Cellulose acetate electrophoresis with specific enzymic and chemical degradation procedures indicated that hyaluronic acid (83%) and heparan sulfate (14%) were the major glycosaminoglycans synthesized by the epidermis when pig ear skin slices were cultured in the presence of D-[3H]glucosamine and 35SO42-, 81% and 50%, respectively, of the total amount of each epidermal glycosaminoglycan was extracellular. Total epidermal glycosaminoglycan synthesis decreased by 50% after 5 days in culture. When the epidermis was cultured in the absence of the dermis the synthesis of hyaluronic acid was reduced considerably. The synthesis of sulfated glycosaminoglycans was essentially unaffected by the absence of the dermis. All-trans-retinoic acid 10-5 M stimulated the synthesis of hyaluronic acid and to a lesser extent sulfated glycosaminoglycans, whether the dermis was absent or present during culture. Hyaluronic acid may play an important role in some aspects of epidermal differentiation.