EXPRESSION OF AN ARABIDOPSIS CRYPTOCHROME GENE IN TRANSGENIC TOBACCO RESULTS IN HYPERSENSITIVITY TO BLUE, UV-A, AND GREEN LIGHT
EXPRESSION OF AN ARABIDOPSIS CRYPTOCHROME GENE IN TRANSGENIC TOBACCO RESULTS IN HYPERSENSITIVITY TO BLUE, UV-A, AND GREEN LIGHT
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DOI：
10.1073/pnas.92.18.8423
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发表时间：
1995-08-29
影响因子：
11.1
通讯作者：
CASHMORE, AR
中科院分区：
文献类型：
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作者：
LIN, C;AHMAD, M;CASHMORE, AR
蓝光诱导抑制下胚轴伸长所需的拟南芥 HY4 基因编码具有蓝光光感受器特征的 75 kDa 黄素蛋白 (CRY1)。为了研究这种光感受器在体内介导蓝光响应的机制，我们在转基因烟草中表达了拟南芥 HY4 基因。转基因植物在蓝光、UV-A 和绿光下，而它们在红/远红光或黑暗中与野生型植物没有差异，发现这种表型与 HY4 转基因的过度表达共分离，并且是通量依赖性的。我们得出结论，转基因烟草植物的短下胚轴表型是由于光感受器过度表达而对蓝光、UV-A 和绿光过敏所致，这些观察结果与由此介导的反应的宽作用谱一致。拟南芥中的隐花色素并表明 CRY1 蛋白所需的信号转导机制在不同植物物种中是保守的。此外，这些光响应的水平被认为是由该光感受器的细胞浓度决定的。
The Arabidopsis HY4 gene, required for blue-light-induced inhibition of hypocotyl elongation, encodes a 75-kDa flavoprotein (CRY1) with characteristics of a blue-light photoreceptor, To investigate the mechanism by which this photoreceptor mediates blue-light responses in vivo, we have expressed the Arabidopsis HY4 gene in transgenic tobacco, The transgenic plants exhibited a short-hypocotyl phenotype under blue, UV-A, and green light, whereas they showed no difference from the wild-type plant under red/far-red light or in the dark, This phenotype was found to cosegregate with overexpression of the HY4 transgene and to be fluence dependent, We concluded that the short-hypocotyl phenotype of transgenic tobacco plants was due to hypersensitivity to blue, UV-A, and green light, resulting from overexpression of the photoreceptor, These observations are consistent with the broad action spectrum for responses mediated by this cryptochrome in Arabidopsis and indicate that the machinery for signal transduction required by the CRY1 protein is conserved among different plant species, Furthermore, the level of these photoresponses is seen to be determined by the cellular concentration of this photoreceptor.