Cytokine modulation of retinoic acid-inducible gene-I (RIG-I) expression in human epidermal keratinocytes
Cytokine modulation of retinoic acid-inducible gene-I (RIG-I) expression in human epidermal keratinocytes
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DOI：
10.1016/j.jdermsci.2006.11.003
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发表时间：
2007-02-01
影响因子：
4.6
通讯作者：
Hanada, Katsumi
中科院分区：
文献类型：
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作者：
Kitamura, Hideo;Matsuzaki, Yasushi;Hanada, Katsumi
背景：视黄酸诱导基因-1（RIG-1）是DExH/D盒家族蛋白的成员，被指定为假定的RNA解旋酶，在响应多种RNA病毒的基因表达和细胞功能中发挥多种作用。目的：本研究旨在探讨干扰素（IFN）-γ和肿瘤坏死因子（TNF）-α对RIG-I表达的影响 人角质形成细胞中RIG-I的表达以及寻常型银屑病皮损中RIG-I的表达，其中IFN-γ和TNF-α被认为参与其发病机制。方法：通过逆转录聚合酶链反应（RT-PCR）和Western blotting分析RIG-1 mRNA和蛋白的表达。在银屑病皮肤切片上检查RIG-1的免疫组织化学染色。结果：在IFN-γ或TNF-α存在的情况下，HaCaT角质形成细胞中RIG-1 mRNA和蛋白的水平上调。免疫组织化学检测显示，在银屑病皮肤的棘层和基底层的细胞质中检测到了 RIG-1。结论：我们的结果表明，RIG-1 可能不仅作为 RNA 解旋酶发挥作用，而且还作为炎症性皮肤病（例如寻常型银屑病）中细胞因子网络的介质。 (c) 2006 年日本皮肤病研究学会。由爱思唯尔爱尔兰有限公司出版。保留所有权利。
Background: Retinoic acid-inducible gene-1 (RIG-1) is a member of the DExH/D box family proteins and designated as a putative RNA helicase, which plays various roles in gene expression and cellular functions in response to a variety of RNA viruses.Objective: The present study was designed to investigate the effects of interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha on RIG-I expression in human keratinocytes, and the expression of RIG-I in skin lesions of psoriasis vulgaris, in which IFN-gamma and TNF-alpha are considered to be involved in its pathogenesis.Methods: The mRNA and protein expression of RIG-1 was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Immunohistochemical staining of RIG-1 was examined on psoriatic skin section.Results: The levels of RIG-1 mRNA and protein were upregulated in HaCaT keratinocytes in the presence of IFN-gamma or TNF-alpha. Immunohistochemically, RIG-1 was detected in the cytoplasm of the spinous and basal layers of psoriatic skin.Conclusion: Our results suggest that RIG-1 might operate not only as a RNA helicase but also as a mediator of the cytokine network in the inflammatory skin diseases, such as psoriasis vulgaris. (c) 2006 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.