Site-specific functionalization of RNA molecules by an unnatural base pair transcription system via click chemistry
Site-specific functionalization of RNA molecules by an unnatural base pair transcription system via click chemistry
复制标题
DOI：
10.1039/c2cc36293g
复制
发表时间：
2012-01-01
影响因子：
4.9
通讯作者：
Hirao, Ichiro
中科院分区：
文献类型：
--
作者：
Ishizuka, Takumi;Kimoto, Michiko;Hirao, Ichiro
针对基因序列扩展的新生物技术，我们开发了一种高效的大RNA分子位点特异性标记方法。非自然碱基对转录和点击化学转录后修饰的结合使得简单的RNA标记在所需的位置上有各种各样的功能基团，在一个锅的反应。
Toward new biotechnology by genetic alphabet expansion, we developed an efficient site-specific labeling method for large RNA molecules. The combination of unnatural base pair transcription and post-transcriptional modification by click chemistry enables simple RNA labeling with a wide variety of functional groups at desired positions, in a one-pot reaction.