Establishment of plant regeneration system for Cucumis melon cv. GT-1
Establishment of plant regeneration system for Cucumis melon cv. GT-1
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作者：
Hu Yong-hong
为了建立稳定、高效的甜瓜植株再生体系，对甜瓜植株进行了再生。 GT-1 在 16 种培养基上进行了研究。将5日龄无菌苗切下的子叶外植体在含有1.0mg/L 6-BA的MS培养基上黑暗培养3天，然后在光照条件下进一步培养以诱导不定芽。将由此产生的芽簇转移至补充有0.05mg/L 6-BA的MS培养基上。将无根伸长芽转移至无激素MS培养基上，诱导生根，获得完整植株。通过该方案，不定芽诱导率为100%，每个外植体平均产生14个健康完整植株。该研究为甜瓜基因转化奠定了良好的基础。
In order to establish a stable and efficient plant regeneration system for Cucumis melon, plant regeneration of C. melon cv. GT-1 was studied on 16 culture mediums. The cotyledon explants excised from 5-day-old germfree seedlings were cultured on the MS medium containing 1.0 mg/L 6-BA in darkness for 3 days, and then further cultured under light condition for induction of adventitious buds. Bud clusters thus generated were transferred onto the MS medium supplemented with 0.05 mg/L 6-BA. The rootless elongated shoots were then transferred to hormone-free MS medium,on which roots were induced and complete plants were obtained. Through the protocol,an adventitious bud induction rate of 100% was obtained and an average of 14 healthy complete plantlets were generated from each explant. The study laid a good foundation for gene transformation of C. melon.