Dark brown is the more virulent of the switch phenotypes of Candida glabrata.
Dark brown is the more virulent of the switch phenotypes of Candida glabrata.
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深棕色是光滑念珠菌转换表型中毒性最强的。
DOI：
10.1099/mic.0.2008/020578-0
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Soll,DavidR
中科院分区：
文献类型：
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作者：
Srikantha,Thyagarajan;Daniels,KarlaJ;Wu,Wei;Lockhart,ShawnR;Yi,Song;Sahni,Nidhi;Ma,Ning;Soll,DavidR
光滑念珠菌在包括深棕色（DB）、浅棕色（LB）和白色（Wh）的表型之间进行可逆的高频核心转换。这些表型又可以转换为不规则皱纹（IWr）表型。然而，天然分离株主要表达DB表型，这导致了它比其他开关表型具有定殖优势的假设。使用小鼠模型的全身感染，结果支持这一假设。DB在小鼠模型中的两个主要靶器官（脾脏和肝脏）的定殖中具有优于其他开关表型的优势。一项时间进程研究表明，宿主注射后主要靶器官的定植发生非常迅速（2小时内），并且DB对脾脏和肝脏定植的优势是即时的。DB优势在从脾、肝和肾清除期间以及在延迟的短暂脑定殖期间保持。这些结果表明，DB具有比其他开关表型的定殖优势，并且由定殖群体表达的开关表型因此在毒力中起着重要作用。因此，在体内和体外研究中必须考虑转换。光叶草毒力
Candida glabrata undergoes reversible, high-frequency core switching between phenotypes that include dark brown (DB), light brown (LB) and white (Wh). These phenotypes in turn can switch to the irregular wrinkle (IWr) phenotype. Natural isolates, however, express predominantly the DB phenotype, leading to the hypothesis that it has a colonization advantage over the other switch phenotypes. Using the mouse model of systemic infection, results are presented which support this hypothesis. DB has an advantage over other switch phenotypes in colonizing the two major target organs in the mouse model, the spleen and liver. A time-course study reveals that colonization of the major target organs occurs very rapidly (within 2 h) after host injection, and that the DB advantage for spleen and liver colonization is immediate. The DB advantage is maintained during clearing from spleen, liver and kidneys, and during delayed transient brain colonization. These results demonstrate that DB has a colonization advantage over other switch phenotypes, and that the switch phenotype expressed by a colonizing population therefore plays a fundamental role in virulence. It is therefore essential that switching be considered in both in vivo and in vitro studies of C. glabrata virulence.