Dispersion versus absorption (DISPA) method for automatic phasing of Fourier transform ion cyclotron resonance mass spectra.
Dispersion versus absorption (DISPA) method for automatic phasing of Fourier transform ion cyclotron resonance mass spectra.
复制标题
用于傅里叶变换离子回旋共振质谱自动定相的色散与吸收 (DISPA) 方法。
DOI：
10.1002/rcm.1290010209
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Marshall,AG
中科院分区：
文献类型：
--
作者：
Craig,EC;Santos,I;Marshall,AG
傅里叶变换离子回旋共振质谱法已经在全球50多个实验室中使用。在最近的评论中列出的FTACR的许多优点中，最吸引人的可能是它在大约40 u时的潜在质量分辨率（例如高达2x 10'）。众所周知，FTIICR质量分辨率与施加的磁场强度成正比，与m/z和压力成反比。较少被广泛认识到的是，对于给定的磁场强度、m/z和压力，仅通过相位校正光谱，FT/ICR质量分辨率可以被增强高达两倍。然而，相位校正需要测量频谱中几个频率处的相位。在本文中，我们提出了一种实用的方法，用于确定相位谱（即相位随频率的变化）在FT/ICR，实验证明了该方法在一个狭窄的质量范围内，并建议扩展到更广泛的质量范围。
Fourier transform ion cyclotron resonance (FTlICR) mass spectrometry ‘.* is already in use in more than fifty laboratories worldwide. Of the many advantages of FTACR listed in recent reviews,’-”’perhaps the most intriguing is its potentially ultrahigh mass resolutioneg up to 2x 10’at about 40u. I’It is well known that FTlICR mass resolution varies directly with applied magnetic field strength and inversely with mlz and pressure. I’It is less widely appreciated that for a given magnetic field strength, mlz, and pressure, FT/ICR mass resolution can be enhanced by up to a factor of two, simply by phase correcting the spectrum. l3 However, phase correction requires the measurement of phase at several frequencies in the spectrum. In this paper, we present a practical method for determining the phase spectrum (ie, variation of phase with frequency) in FT/ICR, demonstrate the method experimentally over a narrow mass range, and suggest extensions to cover a wider mass range.