Circulating osteocyte-derived exosomes contain miRNAs which are enriched in exosomes from MLO-Y4 cells
Circulating osteocyte-derived exosomes contain miRNAs which are enriched in exosomes from MLO-Y4 cells
复制标题
DOI：
10.3892/br.2016.824
复制
发表时间：
2017-02-01
影响因子：
2.3
通讯作者：
Tamura, Masato
中科院分区：
文献类型：
--
作者：
Sato, Mari;Suzuki, Tomohide;Tamura, Masato
骨细胞产生的信号分子被认为是促进骨重塑和其他器官稳态的可溶性因子。然而，据我们所知，目前还没有研究调查骨细胞分泌外泌体的作用。在本研究中，通过消融小鼠骨细胞[osteocyte-less (OL)]来检测血浆循环外泌体的microRNA （miRNA）水平。为了研究骨细胞分泌外泌体的功能，我们提取了来自MLO-Y4细胞的外泌体，并利用miRNA阵列分析和深度测序检测了它们的miRNA表达水平。比较OL小鼠血浆外泌体和mlo - y4来源的外泌体的miRNA表达水平显示，OL小鼠血浆中循环的外泌体miRNA数量减少可能是由骨细胞外泌体分泌减少引起的。这些结果表明，骨细胞分泌含有特征mirna的外泌体，然后在血液中循环，并可能因此将其成分，包括mirna，转移到受体细胞，在其他器官和/或组织中作为信号分子调节生物反应。
Signaling molecules produced by osteocytes have been proposed to serve as soluble factors that contribute to bone remodeling, as well as to homeostasis of other organs. However, to the best of our knowledge, there are currently no studies investigating the role of osteocyte-secreted exosomes. In the present study, ablation of osteocytes in mice [osteocyte-less (OL)] was used to examine the microRNA (miRNA) levels of plasma-circulating exosomes. In order to investigate the function of osteocyte-secreted exosomes, exosomes derived from MLO-Y4 cells were extracted and their miRNA expression levels were examined using miRNA array analysis and deep sequencing. Comparison of miRNA expression levels between plasma exosomes from OL mouse plasma and MLO-Y4-derived exosomes revealed that decreases in the number of miRNAs from exosomes circulating in the OL mouse plasma may be caused by a decrease in secretion of exosomes from osteocytes. These results suggest that osteocytes secrete exosomes containing characterized miRNAs and then circulate in the blood, and may thus transfer their components, including miRNAs, to recipient cells where they function as signaling molecules in other organs and/ or tissues to regulate biological responses.