Analysis of RNA Sequencing Data Using CLC Genomics Workbench.
Analysis of RNA Sequencing Data Using CLC Genomics Workbench.
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DOI：
10.1007/978-1-0716-0223-2_4
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发表时间：
2020-01-01
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影响因子：
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通讯作者：
Di, Y Peter
中科院分区：
文献类型：
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作者：
Liu, Chia-Hsin;Di, Y Peter
RNA测序（RNA-seq）是最近开发的一种利用下一代测序（NGS）技术进行转录组分析的方法。研究表明，RNA-seq提供了转录水平及其异构体的精确测量，这对解决复杂的转录组是有用的。此外，越来越多的公开测序数据集和不断降低的测序成本促进了RNA-seq在假设生成研究中的使用。在本章中，我们演示了如何使用CLC基因组工作台软件分析RNA-seq数据并生成可解释的结果，并使用独创性途径分析（IPA）进行下游途径分析。
RNA sequencing (RNA-seq) is a recently developed approach to perform transcriptome profiling using next-generation sequencing (NGS) technologies. Studies have shown that RNA-seq provides accurate measurement of transcript levels as well as their isoforms, which is useful to address complex transcriptomes. In addition, the increasing publicly available sequencing datasets and decreasing sequencing cost promote the use of RNA-seq for hypothesis-generating studies. In this chapter, we demonstrate how to analyze RNA-seq data and generate interpretable results using CLC genomic workbench software and perform the downstream pathway analysis using ingenuity pathway analysis (IPA).