Micro-channel fabrication by femtosecond laser to arrange neuronal cells on multi-electrode arrays
Micro-channel fabrication by femtosecond laser to arrange neuronal cells on multi-electrode arrays
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DOI：
10.1007/s00339-010-5836-4
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发表时间：
2010-06
期刊：
影响因子：
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通讯作者：
C. Hosokawa;S. Kudoh;Mariko Suzuki;A. Kiyohara;Y. Hosokawa;K. Okano;H. Masuhara;T. Taguchi
中科院分区：
文献类型：
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作者：
C. Hosokawa;S. Kudoh;Mariko Suzuki;A. Kiyohara;Y. Hosokawa;K. Okano;H. Masuhara;T. Taguchi
我们提出并证明了微通道制造飞秒激光加工安排神经元细胞的多电极阵列（MEA）。将神经元细胞接种于微通道中，神经元突起沿着通道伸长，最终在微通道上形成网状结构。通过电生理系统评价MEA上神经元网络的自发电活动，表明图案化网络沿着通道被功能性激活沿着。本方法适用于分析人工图案化的活神经元细胞的网络动力学。
We propose and demonstrate micro-channel fabrication by femtosecond-laser processing to arrange neuronal cells on multi-electrode arrays (MEAs). When neuronal cells were seeded in the micro-channels on MEAs, they elongated their neurites along the channel and finally formed the network patterned on MEAs. The spontaneous electrical activity of the neuronal networks on MEAs was evaluated by using electrophysiological systems, indicating that patterned network was functionally activated along the channels. The present method is applicable to analyze network dynamics of living neuronal cells patterned artificially.