Association between the SIRT1 mRNA Expression and Acute Coronary Syndrome
Association between the SIRT1 mRNA Expression and Acute Coronary Syndrome
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SIRT1 mRNA 表达与急性冠状动脉综合征之间的关联。
DOI：
10.5551/jat.24844
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发表时间：
2015-01-01
影响因子：
4.4
通讯作者：
Gu, Dongfeng
中科院分区：
文献类型：
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作者：
Hu, Yongyan;Wang, Laiyuan;Gu, Dongfeng
目的：沉默交配型信息调节因子2同源物1（SIRT1）在血管生物学中作为动脉粥样硬化保护因子发挥作用，SIRT1的遗传变异与几个人群中的冠状动脉钙化和2型糖尿病相关。本研究旨在探讨SIRT1基因mRNA表达水平与单核苷酸多态性（SNPs）在急性冠状动脉综合征（ACS）发病中的关系。结果：与对照组相比，急性心肌梗死（AMI）、不稳定型心绞痛（UA）和ACS患者SIRT1基因mRNA表达水平均显著降低。这些结果在另一个独立的队列中也得到了证实（AMI，p <0.05）。
Aim: Silent mating type information regulator 2 homolog 1 (SIRT1) functions as an atheroprotective factor in vascular biology, and genetic variations in SIRT1 are associated with coronary artery calcification and type 2 diabetes in several populations. In this study, we investigated the relationship between the mRNA expression levels of the SIRT1 gene and single nucleotide polymorphisms (SNPs) in the context of acute coronary syndrome (ACS).Methods: Whole-genome expression microarray and real-time PCR techniques were used to detect the gene expression levels, and Western blotting was performed to determine the protein expression level. The four selected SNPs were genotyped in a Taqman genotyping platform.Results: Compared with that observed in the controls, the mRNA expression levels of the SIRT1 gene in the microarray study were significantly lower in the acute myocardial infarction (AMI), unstable angina (UA) and overall ACS patients. These results were replicated in another independent cohort with respect to the mRNA (AMI, p