Overlap in the distribution of TrkB immunoreactivity and retinohypothalamic tract innervation of the rat suprachiasmatic nucleus
Overlap in the distribution of TrkB immunoreactivity and retinohypothalamic tract innervation of the rat suprachiasmatic nucleus
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DOI：
10.1016/j.neulet.2004.11.076
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发表时间：
2005-03-16
影响因子：
2.5
通讯作者：
Earnest, DJ
中科院分区：
文献类型：
--
作者：
Allen, GC;Earnest, DJ
脑源性神经营养因子（BDNF）可能通过调节视网膜下丘脑束（RHT）的输入来调节下丘脑视交叉上核（SCN）生物钟对光的昼夜敏感性。在本研究中，BDNF的同源受体，TrkB酪氨酸激酶，在RHT纤维和SCN的大鼠的解剖分布进行了分析，使用联合免疫组织化学和顺行追踪方法。TrkB受体的荧光免疫染色在整个SCN的纤维和细胞体中是明显的。双标记分析显示，有大量的重叠分布的Trk B免疫染色和霍乱毒素亚单位B（CT B）-标记内的RHT终端和纤维从视交叉投射到腹外侧SCN。目前的结果表明，RHT纤维可能表达TrkB受体，从而提供了一个旁分泌的BDNF介导的调节光输入到SCN的目标。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
Brain-derived neurotrophic factor (BDNF) may regulate the circadian sensitivity of the clock in the hypothalamic suprachiasmatic nucleus (SCN) to light, possibly by modulating retinohypothalamic tract (RHT) input. In the present study, the anatomical distribution of the cognate receptor for BDNF, the TrkB tyrosine kinase, in RHT fibers and the SCN of rats was analyzed using combined immunohistochemical and anterograde tracing methods. Fluorescent immunostaining for the TrkB receptor was evident in fibers and cell bodies throughout the SCN. Dual labeling analysis revealed that there was substantial overlap in the distribution of TrkB immunostaining and cholera toxin subunit B (CTB)-labeling within RHT terminals and fibers projecting from the optic chiasm to the ventrolateral SCN. The present results suggest that RHT fibers may express TrkB receptors and thus provide a paracrine target for BDNF-mediated regulation of photic input to the SCN. (C) 2004 Elsevier Ireland Ltd. All rights reserved.