An enhancer trap in the ascidian Ciona intestinalis identifies enhancers of its Musashi orthologous gene
An enhancer trap in the ascidian Ciona intestinalis identifies enhancers of its Musashi orthologous gene
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DOI：
10.1016/j.ydbio.2004.08.029
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发表时间：
2004-11-15
影响因子：
2.7
通讯作者：
Sasakura, Y
中科院分区：
文献类型：
--
作者：
Awazu, S;Sasaki, A;Sasakura, Y
在果蝇中建立的增强子陷阱技术是一种非常复杂的工具。然而，尽管它很有用，但在其他动物中很少有关于增强子陷阱的报道。海鞘是发育生物学的一个极好的实验系统，为发育遗传学研究提供了良好的材料。近年来，C.已经使用Tc 1/mariner超家族转座子Minos实现了转座。在该研究过程中，分离了一个显示出与其他系不同的GFP表达模式的Minos插入系。一个有趣的可能性是，在这条线上发生了增强子陷阱事件。在这里，我们表明，在Ci-Musashi基因的Minos插入负责改变GFP表达。Ci-Musashi显示出与GFP相似的表达模式。此外，Ci-Musashi的内含子具有增强子活性，可以改变附近基因的表达模式，使其类似于该品系中GFP的表达模式。这些结果清楚地表明，在C.好吧(C)2004爱思唯尔公司All rights reserved.
The enhancer trap technique, established in Drosophila melanogaster, is a very sophisticated tool. Despite its usefulness, however, there have been very few reports on enhancer traps in other animals. The ascidian Ciona intestinalis, a splendid experimental system for developmental biology, provides good material for developmental genetics. Recently, germline transgenesis of C. intestinalis has been achieved using the Tc1/mariner superfamily transposon Minos. During the course of that study, one Minos insertion line that showed a different GFP expression pattern from other lines was isolated. One fascinating possibility is that an enhancer trap event occurred in this line. Here we show that a Minos insertion in the Ci-Musashi gene was responsible for the altered GFP expression. Ci-Musashi showed a similar expression pattern to GFP. In addition, introns of Ci-Musashi have enhancer activity that can alter the expression pattern of nearby genes to resemble that of GFP in this line. These results clearly demonstrate that an enhancer trap event that entrapped enhancers of Ci-Musashi occurred in C. intestinalis. (C) 2004 Elsevier Inc. All rights reserved.