A 60-KDA PLASMODIUM-FALCIPARUM PROTEIN AT THE MOVING JUNCTION FORMED BETWEEN MEROZOITE AND ERYTHROCYTE DURING INVASION
A 60-KDA PLASMODIUM-FALCIPARUM PROTEIN AT THE MOVING JUNCTION FORMED BETWEEN MEROZOITE AND ERYTHROCYTE DURING INVASION
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DOI：
10.1016/0166-6851(89)90190-4
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发表时间：
1989-09-01
影响因子：
1.5
通讯作者：
MILLER, LH
中科院分区：
文献类型：
--
作者：
KLOTZ, FW;HADLEY, TJ;MILLER, LH
疟原虫裂殖子侵入红细胞需要在红细胞和裂殖子膜之间形成连接。附着连接最初形成于裂殖子的顶端区域。然后随着入侵的进行，它移动到裂殖子的后部。针对恶性疟原虫的60-kDa裂殖子蛋白（称为MCP-1的裂殖子帽蛋白1）的单克隆抗体以类似于移动连接的免疫荧光模式反应。双色免疫荧光显示MCP-1定位于裂殖子顶端与红细胞的结合部位。在入侵过程中，MCP-1分离和迁移周围的裂殖子的口寄生液泡。在新侵入的红细胞中，MCP-1持续存在于最靠近红细胞膜的年轻寄生虫的极点，表明其前后运动。MCP-1在恶性疟原虫的FCR-3、Camp和7 G8菌株中没有表现出分子量的变化，并且表位在所研究的恶性疟原虫菌株中是不变的。我们的结论是，MCP-1可能参与裂殖子入侵红细胞，促进附件或运动的连接沿着寄生虫的细胞骨架网络。
Invasion of erythrocytes by malaria merozoites requires the formation of a junction of attachment between erythrocyte and merozoite membranes. The attachment junction initially forms at the apical region of the metrozoite. It then moves around to the posterior of the merozoite as invasion proceeds. A monoclonal antibody against a 60-kDa merozoite protein (termed MCP-1 for merozoite capping protein 1) of Plasmodium falciparum reacts in an immunofluorescence pattern resembling the moving junction. By two-color immunofluorescence, MCP-1 was located at the attachment site formed between the merozoite apical region and erythrocyte. During invasion, MCP-1 separated and migrated around merozoites at the orifice of the parasitophorous vacuole. In newly-invaded erythrocytes, MCP-1 persisted at the pole of the young parasite nearest the erythrocyte membrane, suggesting its anterior-to-posterior movement. MCP-1 exhibited no variability in molecular mass among the FCR-3, Camp and 7G8 strains of P. falciparum, and the epitope was invariant in the P. falciparum strains studied. We conclude that MCP-1 may participate in merozoite invasion of erythrocytes by facilating attachment or movement of the junction along the parasite cytoskeletal network.