The early entry of Al into cells of intact soybean roots - A comparison of three developmental root regions using secondary ion mass spectrometry imaging
The early entry of Al into cells of intact soybean roots - A comparison of three developmental root regions using secondary ion mass spectrometry imaging
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DOI：
10.1104/pp.112.3.1289
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发表时间：
1996-11-01
期刊：
影响因子：
7.4
通讯作者：
Linton, RW
中科院分区：
文献类型：
--
作者：
Lazof, DB;Goldsmith, JG;Linton, RW
采用二次离子质谱法比较了铝敏感大豆基因型初生根三个发育区域中铝的定位。在cryosections后4小时暴露于38亩M [Al 3 +]，铝渗透到整个根，并进入中柱在所有三个地区。尽管最大的局部铝浓度始终在根周，但每个区域的大部分铝都积累在皮质细胞中。很明显，二次离子质谱Al-27（+）质量信号分布在整个细胞内区域，在细胞壁中并不特别强烈。包括一些细胞壁的铝水平的测定在整个根半径需要这些作为细胞内铝的最低估计。细胞内铝的总积累为每个区域为60，73，和210 nmol g（-1）鲜重后4小时，随着根的发展。早期的代谢反应，外部铝，包括那些已被报道的根内深处和成熟地区，可能会直接从细胞内铝。这些反应可能包括离子运输事件在成熟的根或事件与侧根出现，以及根尖内的事件。
Al localization was compared in three developmental regions of primary root of an Al-sensitive soybean (Glycine max) genotype using secondary ion mass spectrometry. In cryosections obtained after a 4-h exposure to 38 mu M [Al3+], Al had penetrated across the root and into the stele in all three regions. Although the greatest localized Al concentration was consistently at the root periphery, the majority of the Al in each region had accumulated in cortical cells. It was apparent that the secondary ion mass spectrometry Al-27(+) mass signal was spread throughout the intracellular area and was not particularly intense in the cell wall. Inclusion of some cell wall in determinations of the Al levels across the root radius necessitated that these serve as minimal estimates for intracellular Al. Total accumulation of intracellular Al for each region was 60, 73, and 210 nmol g(-1) fresh weight after 4 h, increasing with root development. Early metabolic responses to external Al, including those that have been reported deep inside the root and in mature regions, might result directly from intracellular Al. These responses might include ion transport events at the endodermis of mature roots or events associated with lateral root emergence, as well as events within the root tip.