End-to-side nerve repair induces nuclear translocation of activating transcription factor 3
End-to-side nerve repair induces nuclear translocation of activating transcription factor 3
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DOI：
10.1080/02844310600999956
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发表时间：
2006-12-01
影响因子：
--
通讯作者：
Kanje, Martin
中科院分区：
文献类型：
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作者：
Bontioti, Eleana;Dahlin, Lars B.;Kanje, Martin
我们想知道是否有三种不同的操作方式：一块肌肉或神经与神经平行；缝合线：在神经外开的窗口，或插入神经的缝合线，或两者兼而有之；将神经片与肌皮神经或坐骨神经的神经外窗端侧缝合，在神经元和非神经元细胞中激活转录因子3 （ATF3）。操作1周后免疫细胞化学检测细胞活化标志物ATF3。与神经平行的神经或肌肉不会在神经中局部诱导ATF3，仅在神经元中很少诱导ATF3。相比之下，神经外窗或缝线插入，或两者兼而有之，无论是否将神经端侧连接，均可诱导ATF3的强烈表达。我们的结论是，与端侧神经修复相关的周围神经干损伤激活了神经元和非神经元细胞，并可能促进轴突在端侧连接的神经中萌发。
We wanted to find out if any of three different types of manipulations: a piece of muscle or nerve put parallel to a nerve; an epineurial window made, or sutures inserted into a nerve, or both; or pieces of nerve sutured to an epineurial window end-to-side to the musculocutaneous or sciatic nerve, resulted in activation of activating transcription factor 3 ( ATF3) in neurons and in non-neuronal cells. ATF3, a marker of cell activation, was investigated by immunocytochemistry one week after manipulation. A piece of nerve or muscle parallel to a nerve did not induce ATF3 locally in the nerve and induced ATF3 only rarely in neurons. In contrast, an epineurial window or insertion of sutures, or both, with or without attachment of a piece of nerve placed end-to-side, induced robust ATF3 expression. We conclude that an injury to a peripheral nerve trunk associated with end-to-side nerve repair, activates neurons and non-neuronal cells and may contribute to sprouting of axons into the nerve attached end-to-side.