Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method
Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method
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DOI：
10.52568/000010
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发表时间：
2021
影响因子：
0.7
通讯作者：
Cheng Zhao Cheng Zhao-Cheng-Zhao;Qing Song Yao Qing Song Yao-Qing-Song-Yao;Can Wang Can Wang-Can-Wang;Mu Tan Luo Mu Tan Luo-Mu-Tan-Luo;Chao Huang Chao Huang-Chao-Huang;Xue Fang Chen Xue Fang Chen-Xue-Fang-Chen;Lan Lan Tian Lan Lan Tian-Lan-Lan-Tian-Lan;Qian Lin Huang Qian Lin Huang-Qian-Lin-Huang;Lian Xiong Lian Xiong-Lian-Xiong;Hai Long Li Hai Long Li-Hai-Long-Li;Xin De Chen Xin De Chen-Xin-De-Chen
中科院分区：
文献类型：
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作者：
Cheng Zhao Cheng Zhao-Cheng-Zhao;Qing Song Yao Qing Song Yao-Qing-Song-Yao;Can Wang Can Wang-Can-Wang;Mu Tan Luo Mu Tan Luo-Mu-Tan-Luo;Chao Huang Chao Huang-Chao-Huang;Xue Fang Chen Xue Fang Chen-Xue-Fang-Chen;Lan Lan Tian Lan Lan Tian-Lan-Lan-Tian-Lan;Qian Lin Huang Qian Lin Huang-Qian-Lin-Huang;Lian Xiong Lian Xiong-Lian-Xiong;Hai Long Li Hai Long Li-Hai-Long-Li;Xin De Chen Xin De Chen-Xin-De-Chen
为了避免油脂酵母油脂含量测定的复杂步骤，特别是在木质纤维素水解液中积累微生物油脂的油脂酵母，采用尼罗红作为荧光染料的荧光法测定了油脂酵母皮氏丝孢酵母的油脂含量。将常规重量法测定的油脂含量与荧光强度进行拟合，建立了荧光法测定油脂含量的方法。在所测得的脂肪含量范围内，拟合曲线呈良好的线性关系（R2=0.95），表明该方法适用于T.在模拟介质中的扩散。为了评价该方法在以木质纤维素酸水解物为底物的油脂发酵中的适用性，T。以玉米芯酸水解液和稻草酸水解液为培养介质，比较了荧光法和重量法测定脂肪含量的差异。结果表明，两种方法测得的脂肪含量相近，因此，该方法在木质纤维素酸水解液脂肪发酵中具有良好的应用前景。
To avoid complex procedures in measurement of lipid content of oleaginous yeast especially for that can accumulate microbial lipid in lignocellulosic hydrolysates, fluorescent method using Nile Red as fluorescent dye was applied to measure lipid content of oleaginous yeast Trichosporon dermatis. The fluorescent method was built by fitting of lipid content identified by both conventional gravimetric method and fluorescence intensity of oleaginous yeast. Within the range of lipid content measured, the fitting curves showed linear relationship with good correlation coefficient (R2=0.95), showing this method is suitable for measuring lipid content of T. dermatis in the simulated medium. To evaluate the applicability of this method for lipid fermentation using lignocellulosic acid hydrolysates as substrate, T. dermatis was cultured in corncob acid hydrolysate and rice straw acid hydrolysate and then its lipid content measured by both fluorescent method and gravimetric method were compared. The results showed that the lipid content measured by these two methods were close, therefore, this method was promising for the application in lipid fermentation in lignocellulosic acid hydrolysates.