High-shear stress sensitizes platelets to subsequent low-shear conditions.
High-shear stress sensitizes platelets to subsequent low-shear conditions.
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DOI：
10.1007/s10439-010-9936-2
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发表时间：
2010-04
影响因子：
3.8
通讯作者：
Jesty, Jolyon
中科院分区：
文献类型：
--
作者：
Sheriff, Jawaad;Bluestein, Danny;Girdhar, Gaurav;Jesty, Jolyon
接受机械心脏瓣膜植入术的患者受到血流诱导的血栓栓塞并发症的困扰，这些并发症无疑是由血小板激活引起的。受影响区域内或周围的流场包括短暂暴露在100至1000达因/平方厘米的病理性高剪应力下。尽管已知高切变可直接激活血小板，但它们随后的行为尚不清楚。我们假设，血小板的高切后激活行为与理解血液循环人工心血管装置相关的血栓形成风险的增加特别相关。纯化的血小板暴露于短时间(5-40 S)的高切应力，然后暴露于较长时间(15-60分钟)的低切应力。使用基于凝血酶原酶的检测来测量它们的激活状态。短暂暴露于初始高切应力(例如，40 S为60达因/厘米~2)的血小板会有少量激活，但这项研究表明，它们现在被敏化，当暴露于随后的低切应力时，它们的激活速度至少是最初未暴露于高切变的血小板的20倍。结果表明，在体外暴露于超过高切应力阈值的血小板，在正常的心血管循环条件下为随后的激活做好了准备，并且它们不能从最初的高切应力损伤中恢复。
Individuals with mechanical heart valve implants are plagued by flow-induced thromboembolic complications, which are undoubtedly caused by platelet activation. Flow fields in or around the affected regions involve brief exposure to pathologically high-shear stresses on the order of 100 to 1000 dyne/cm2. Although high shear is known to activate platelets directly, their subsequent behavior is not known. We hypothesize that the post-high-shear activation behavior of platelets is particularly relevant in understanding the increased thrombotic risk associated with blood-recirculating prosthetic cardiovascular devices. Purified platelets were exposed to brief (5–40 s) periods of high-shear stress, and then exposed to longer periods (15–60 min) of low shear. Their activation state was measured using a prothrombinase-based assay. Platelets briefly exposed to an initial high-shear stress (e.g., 60 dyne/cm2 for 40 s) activate a little, but this study shows that they are now sensitized, and when exposed to subsequent low shear stress, they activate at least 20-fold faster than platelets not initially exposed to high shear. The results show that platelets in vitro exposed beyond a threshold of high-shear stress are primed for subsequent activation under normal cardiovascular circulation conditions, and they do not recover from the initial high-shear insult.
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影响因子：
2.4
作者：
Bray, Paul F.
通讯作者：
Bray, Paul F.
影响因子：
3.3
作者：
Jesty, J;Yin, W;Bluestein, D
通讯作者：
Bluestein, D
影响因子：
4.8
作者：
Bahou, WF;Scudder, L;Jesty, J
通讯作者：
Jesty, J
影响因子：
20.3
作者：
Miyazaki, Y;Nomura, S;Fukuhara, S
通讯作者：
Fukuhara, S
DOI：
10.1161/01.atv.0000229658.76797.30
发表时间：
2006-08-01
影响因子：
8.7
作者：
Hathcock, James J.
通讯作者：
Hathcock, James J.