Single-nucleotide repeat analysis for subtyping Bacillus anthracis isolates
Single-nucleotide repeat analysis for subtyping Bacillus anthracis isolates
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DOI：
10.1128/jcm.44.3.777-782.2006
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发表时间：
2006-03-01
影响因子：
9.4
通讯作者：
Bader, D
中科院分区：
文献类型：
--
作者：
Stratilo, CW;Lewis, CT;Bader, D
单核苷酸重复序列（SNRs）是一种数目可变的串联重复序列，具有很高的突变率。在暴发情况下，利用具有非常高突变率的区域的标记系统（例如SNRs）的使用允许区分具有极低遗传多样性水平的分离物。本文报道了炭疽芽孢杆菌SNR位点的鉴定和分析。在计算机上选择SNR基因座，并使用具有最高多样性的基因座来设计和测试针对许多B的基因座特异性引物。炭疽菌株具有相同的多位点可变数目串联重复序列分析（MLVA）基因型。识别了允许具有相同MLVA基因型的菌株彼此区分的SNR标记。由此产生的SNR标记系统可用作自然爆发或生物恐怖事件中的分子流行病学工具，提供区分非常密切相关的分离株的最佳机会。
Single-nucleotide repeats (SNRs) are variable-number tandem repeats that display very high mutation rates. In an outbreak situation, the use of a marker system that exploits regions with very high mutation rates, such as SNRs, allows the differentiation of isolates with extremely low levels of genetic diversity. This report describes the identification and analysis of SNR loci of Bacillus anthracis. SNR loci were selected in silico, and the loci with the highest diversity were used to design and test locus-specific primers against a number of B. anthracis strains with the same multilocus variable-number tandem repeat analysis (MLVA) genotype. SNR markers that allowed strains with the same MLVA genotype to be differentiated from each other were identified. The resulting SNR marker system can be used as a molecular epidemiological tool in a natural outbreak or bioterrorism event, offering the best chance of distinguishing very closely related isolates.