MEAT SCIENCE AND MUSCLE BIOLOGY SYMPOSIUM: The influence of extracellular matrix on intramuscular and extramuscular adipogenesis
MEAT SCIENCE AND MUSCLE BIOLOGY SYMPOSIUM: The influence of extracellular matrix on intramuscular and extramuscular adipogenesis
复制标题
DOI：
10.2527/jas.2011-4616
复制
发表时间：
2012-03-01
影响因子：
3.3
通讯作者：
Hausman, G. J.
中科院分区：
文献类型：
--
作者：
Hausman, G. J.
细胞外基质（ECM）和特定的ECM组分可以对细胞生长、发育和表型产生重大影响。本文就细胞外基质及其成分在体内、外对脂肪形成的影响作一综述。Engelbreth-Holm-Swarm基质和层粘连蛋白本身显著增加了猪脂肪组织基质血管细胞无血清原代培养物中前脂肪细胞的附着、扩散和肥大。此外，基质血管细胞的原代培养物显示，前脂肪细胞表达ECM成分后，前脂肪细胞招聘。对胎猪脂肪组织中植物凝集素、IV型胶原和层粘连蛋白的染色表明，在整个胎儿发育过程中，脂肪细胞对层粘连蛋白的反应性很强，并且与发育中的脂肪细胞和脉管系统相似。然而，血管的凝集素结合和IV型胶原的反应性先于脂肪细胞。因此，这些研究表明，ECM，特别是层粘连蛋白可能在前脂肪细胞发育的形态学方面发挥关键作用。胶原合成的特异性抑制剂和调节剂已被用于评估胶原在牛肌内前脂肪细胞（BIP）和其他前脂肪细胞系分化中的作用。BIP细胞的甘油三酯增加被胶原蛋白生物合成的一般抑制剂抑制，而IV型胶原蛋白的特异性抑制剂或调节剂抑制3 T3-L1细胞分化。进一步的研究表明，与I型至IV型胶原相比，V型和VI型胶原在BIP脂肪形成中具有重要和积极的作用。牛肌内脂肪组织的生长可能依赖于脂肪细胞自身新合成和组织化的胶原蛋白。细胞外或ECM蛋白水解在调节脂肪形成中的作用也将在本文中进行综述。基质金属蛋白酶（MMP）家族的许多成员由脂肪细胞表达，并且MMP-9的特异性抑制大大减少体外脂肪形成。MMP和其他蛋白酶可能调节参与前脂肪细胞增殖和分化调节的关键脂肪细胞ECM蛋白的周转。
The extracellular matrix (ECM) and specific ECM components can have a major influence on cell growth, development, and phenotype. The influence of the ECM and ECM components on adipogenesis in vivo and in vitro will be reviewed in this paper. Engelbreth-Holm-Swarm substratum and laminin per se markedly increased attachment, spreading, and hypertrophy of preadipocytes in serum-free primary cultures of porcine adipose tissue stromal-vascular cells. Furthermore, primary cultures of stromal-vascular cells showed that preadipocytes express ECM components after preadipocyte recruitment. Staining for plant lectins, type IV collagen, and laminin in fetal pig adipose tissue demonstrates that adipocyte reactivity for laminin was strong throughout fetal development and was similar for developing adipocytes and vasculature. However, lectin binding and type IV collagen reactivity of blood vessels preceded that for adipocytes. Therefore, these studies indicated that the ECM and in particular laminin may play a critical role in morphological aspects of preadipocyte development. Specific inhibitors and modulators of collagen synthesis have been used to evaluate the role of collagens in the differentiation of bovine intramuscular preadipocytes (BIP) and other preadipocyte cell lines. Triglyceride accretion of BIP cells was inhibited by a general inhibitor of collagen biosynthesis, whereas specific inhibitors or modulators of type IV collagen inhibited 3T3-L1 cell differentiation. Further study revealed that compared with collagens types I to IV, type V and VI collagens have an important and active role in BIP adipogenesis. The growth of intramuscular bovine adipose tissue may be dependent on collagen newly synthesized and organized by the adipocytes per se. The role of extracellular or ECM proteolysis in regulating adipogenesis also will be reviewed in this paper. Many members of the matrix metalloproteinase (MMP) family are expressed by adipocytes, and specific inhibition of MMP-9 greatly reduces adipogenesis in vitro. Possibly, MMP and other proteases regulate turnover of key adipocyte ECM proteins that are involved in the regulation of preadipocyte proliferation and differentiation.