Graphene Oxide Wrapped SERS Tags: Multifunctional Platforms toward Optical Labeling, Photothermal Ablation of Bacteria, and the Monitoring of Killing Effect
Graphene Oxide Wrapped SERS Tags: Multifunctional Platforms toward Optical Labeling, Photothermal Ablation of Bacteria, and the Monitoring of Killing Effect
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氧化石墨烯包裹的 SERS 标签：光学标记、细菌光热消融和杀伤效果监测的多功能平台
DOI：
10.1021/am405396k
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发表时间：
2014-01-22
影响因子：
9.5
通讯作者：
Chen, Lingxin
中科院分区：
文献类型：
--
作者：
Lin, Donghai;Qin, Tianqi;Chen, Lingxin
表面增强拉曼散射（SERS）标签作为一种新型的光学纳米探针，在生物医学成像和光治疗领域的应用越来越受到人们的关注。在这里，我们展示了一种新的原位合成氧化石墨烯包裹金纳米簇SERS标签的策略，通过使用三（2,2'-联吡啶基）钌-(II)氯（红宝石）/氧化石墨烯纳米杂化物作为复杂的拉曼报告蛋白，灵感来自于氧化石墨烯作为各种染料的人工受体的作用。在合成过程中引入氧化石墨烯，为控制SERS标签的几个关键参数提供了系统的解决方案，包括再现性、灵敏度、胶体和信号稳定性。另外一个有趣的热敏SERS特性（SERS强度随温度升高而降低）是由于氧化石墨烯吸附的报告分子的热诱导释放/重新分配而实现的。结合这些特性的协同效应，我们进一步制备了多功能的醛基共轭Au@Rubpy/GO SEAS标签，用于细菌的光学标记和光热消融。实现了革兰氏阳性菌（金黄色葡萄球菌）和革兰氏阴性菌（大肠杆菌）的灵敏拉曼成像，对两种细菌均有满意的光热杀灭效果。我们的结果还显示了SEAS强度降低率、细菌存活率和标签-细菌悬浮液的末端温度之间的相关性，显示了使用SERS测定该标签在光热过程中的抗菌反应的可能性。
As novel optical nanoprobes, surface-enhanced Raman scattering (SERS) tags have drawn growing interests in the application of biomedical imaging and phototherapies. Herein, we demonstrated a novel in situ synthesis strategy for GO wrapped gold nanocluster SERS tags by using a tris(2,2'-bipyridyl)ruthenium-(II) chloride (Rubpy)/GO nanohybrid as a complex Raman reporter, inspired by the role of GO as an artificial receptor for various dyes. The introduction of GO in the synthesis procedure provided systematic solutions for controlling several key parameters of SERS tags, including reproducibility, sensitivity, and colloidal and signal stability. An additional interesting thermal-sensitive SERS property (SERS intensity decreased upon increasing the temperature) was also achieved due to the heat-induced release/redistribution of reporter molecules adsorbed on GO. Combining the synergic effect of these features, we further fabricated multifunctional, aldehyde group conjugated Au@Rubpy/GO SEAS tags for optical labeling and photothermal ablation of bacteria. Sensitive Raman imaging of gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria could be realized, and satisfactory photothermal killing efficacy for both bacteria was achieved. Our results also demonstrated the correlation among the SEAS intensity decrease ratio, bacteria survival rate, and the terminal temperature of the tag-bacteria suspension, showing the possibility to use SERS assay to measure antibacterial response during the photothermal process using this tag.