Molecular characterisation of Turnip mosaic virus isolates from Brassicaceae weeds
Molecular characterisation of Turnip mosaic virus isolates from Brassicaceae weeds
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DOI：
10.1007/s10658-008-9390-2
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发表时间：
2009-05-01
影响因子：
1.8
通讯作者：
Ohshima, Kazusato
中科院分区：
文献类型：
--
作者：
Farzadfar, Shirin;Tomitaka, Yasuhiro;Ohshima, Kazusato
在2003-2008年期间，对伊朗的8个省进行了调查，以寻找芜菁花叶病毒（TuMV）的寄主。从具有病毒样症状的植物中共收集了532个杂草样品。使用特异性抗体通过酶联免疫吸附测定法检测样品中TuMV的存在。在这些测试中，340个样本（64%）被发现感染TuMV。Rapistrum rugosum、Ssymomberium loeselii、S. Irio和Hirschfeldia incana被鉴定为TuMV的兰科杂草宿主，前两种植物被发现是伊朗最重要的杂草宿主。IRN TRa 6和IRN SS 5分离物的基因组RNA全长序列。rugosum和S.罗勒氏病是有决心的。使用不同的重组检测方案，在两种分离株中均未发现重组的证据。系统发育分析表明，IRN TRa 6和IRN SS 5菌株属于一个祖先basal-Brassica组。这项研究首次显示了TuMV的广泛分布和系统发育关系，从杂草在欧亚大陆中部的伊朗。
Eight provinces of Iran were surveyed during 2003-2008 to find Brassicaceae reservoir weed hosts of Turnip mosaic virus (TuMV). A total of 532 weed samples were collected from plants with virus-like symptoms. The samples were tested for the presence of TuMV by enzyme-linked immunosorbent assay using specific antibodies. Among those tested, 340 samples (64%) were found to be infected with TuMV. Rapistrum rugosum, Sisymberium loeselii, S. irio and Hirschfeldia incana were identified as the Brassicaceae weed hosts of TuMV, and the former two plant species were found to be the most important weed hosts for the virus in Iran. The full-length sequences of the genomic RNAs of IRN TRa6 and IRN SS5 isolates from R. rugosum and S. loeselii were determined. No evidence of recombination was found in both isolates using different recombination-detecting programmes. Phylogenetic analyses of the weed isolates with representative isolates from the world showed that the IRN TRa6 and IRN SS5 isolates fell into an ancestral basal-Brassica group. This study shows for the first time the wide distribution and phylogenetic relationships of TuMV from weeds in the mid-Eurasia of Iran.