Usefulness of specific OA biomarkers, thrombin-cleaved osteopontin, in the posterior cruciate ligament OA rabbit model
Usefulness of specific OA biomarkers, thrombin-cleaved osteopontin, in the posterior cruciate ligament OA rabbit model
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特定 OA 生物标志物（凝血酶裂解骨桥蛋白）在后交叉韧带 OA 兔模型中的用途
DOI：
10.1016/j.joca.2012.09.006
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发表时间：
2013-01-01
影响因子：
7
通讯作者：
Lei, G. H.
中科院分区：
文献类型：
--
作者：
Gao, S. G.;Cheng, L.;Lei, G. H.
目的：我们进行了这项研究，以确定滑膜液（SF）中凝血酶裂解骨桥蛋白（OPN）是否代表骨性关节炎（OA）进展的有用标志。方法：对48只兔右膝关节行PCLT。术后4周、8周、16周和24周分别处死，取下关节，对关节软骨进行宏观和组织学评估。用Western blotting测定SF中凝血酶裂解的OPN产物，并用酶联免疫分析法测定其水平。结果：PCLT膝关节术后4周宏观及组织学评分均已升高，且随时间延长而升高。Western blotting显示PCLT膝关节SF中存在凝血酶裂解的OPN。与基线相比，PCLT后SF中凝血酶切割的OPN水平在4周时升高（P < 0.001），在24周时达到峰值（P < 0.00001）。凝血酶切割OPN水平与宏观评分呈显著正相关（8周：rho = 0.695, P = 0.012； 16周：rho = 0.751, P = 0.005； 24周：rho = 0.660, P = 0.020）。此外，凝血酶切割的OPN水平与组织学评分之间也存在相同的相关性（4周：rho = 0.609, P = 0.036； 8周：rho = 0.662, P = 0.019； 16周：rho = 0.827, P = 0.001； 24周：rho = 0.813, P = 0.001）。结论：在这种兔PCLT模型中，SF中凝血酶裂解的OPN水平似乎是OA疾病严重程度和进展的有用标志。2012年，爱思唯尔有限公司代表国际骨关节炎研究学会出版。版权所有。
Objective: We undertook this study to determine whether thrombin-cleaved osteopontin (OPN) in synovial fluid (SF) represents a useful marker of osteoarthritis (OA) progression in the posterior cruciate ligament transection (PCLT) OA rabbit model.Method: PCLT was performed on the right knee joints of 48 rabbits. The rabbits were then sacrificed separately at 4, 8, 16, and 24 weeks post-surgery, when the joint was harvested and macroscopic and histological assessments of articular cartilage were performed. Thrombin-cleaved OPN product in SF was determined using Western blotting and the levels were measured using an enzyme-linked immunoassay.Results: The macroscopic and histological scores for PCLT knees were already elevated 4 weeks after surgery and increased with time. Western blotting showed the presence of thrombin-cleaved OPN in SF from PCLT knees. Thrombin-cleaved OPN levels in SF were elevated at 4 weeks (P < 0.001) and were elevated peaking at 24 weeks (P < 0.00001) after PCLT compared to baseline. A positive significant correlation was found between thrombin-cleaved OPN levels and the macroscopic scores (8 weeks: rho = 0.695, P = 0.012; 16 weeks: rho = 0.751, P = 0.005; 24 weeks: rho = 0.660, P = 0.020). Furthermore, the same correlation was noted between thrombin-cleaved OPN levels and the histological scores (4 weeks: rho = 0.609, P = 0.036; 8 weeks: rho = 0.662, P = 0.019; 16 weeks: rho = 0.827, P = 0.001; 24 weeks: rho = 0.813, P = 0.001).Conclusion: In this rabbit model of PCLT, thrombin-cleaved OPN levels in SF appear to provide a useful marker of OA disease severity and progression. Crown Copyright (C) 2012 Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International. All rights reserved.