Could Alkali Production Be Considered an Approach for Caries Control?
Could Alkali Production Be Considered an Approach for Caries Control?
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DOI：
10.1159/000321139
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发表时间：
2010-01-01
期刊：
影响因子：
4.2
通讯作者：
Magnusson, I.
中科院分区：
文献类型：
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作者：
Gordan, V. V.;Garvan, C. W.;Magnusson, I.
本研究通过病例对照研究，探讨精氨酸脱亚胺酶（ADS）和尿素酶活性与龋病的关系。ADS和尿素酶活性测定牙齿光滑表面龈上菌斑和全唾液样本93名受试者，谁在三个不同的组：无龋（n = 31），龋活跃（n = 30），龋经验（n = 32）。通过定量在含有50 mM盐酸马槟榔碱和50 mM Tris-马来酸盐缓冲液（pH 6.0）的混合物中孵育牙菌斑和唾液样品产生的氨来测量ADS活性。ADS比活性定义为每毫克蛋白质每分钟产生的氨的纳摩尔数。通过定量由50 mM尿素产生的氨来测定脲酶活性。对于细菌鉴定和计数，使用实时qPCR分析。使用Kruskal-Wallis检验比较各组。采用斯皮尔曼相关性分析菌斑代谢活性和细菌关系。结果显示，与龋齿活跃的受试者相比，无龋齿受试者的唾液（1.06 vs. 0.18; p < 0.0001）和牙菌斑样本（1.74 vs. 0.58; p < 0.0001）中精氨酸的氨产量显著更高。无龋受试者斑块中的尿素酶水平约高3倍（p < 0.0001）。虽然有龋和无龋受试者唾液中的脲酶活性较高，但组间无显著差异。版权所有（C）2010 S. Karger AG，巴塞尔
This study investigated the relationship of arginine deiminase (ADS) and urease activities with dental caries through a case-control study. ADS and urease activities were measured in dental smooth-surface supragingival plaque and whole saliva samples from 93 subjects, who were in three different groups: caries-free (n = 31), caries-active (n = 30), and caries-experienced (n = 32). ADS activity was measured by quantification of the ammonia generated from the incubation of plaque and saliva samples in a mixture containing 50 m M arginine-HCl and 50 m M Tris-maleate buffer, pH 6.0. ADS-specific activity was defined as nanomoles of ammonia generated per minute per milligram of protein. Urease activity was determined by quantification of ammonia produced from 50 m M urea. For bacterial identification and enumeration real-time qPCR analysis was used. Groups were compared using Kruskal-Wallis tests. Spearman correlations were used to analyze plaque metabolic activity and bacterial relationships. The results revealed significantly higher ammonia production from arginine in saliva (1.06 vs. 0.18; p < 0.0001) and plaque samples (1.74 vs. 0.58; p < 0.0001) from caries-free subjects compared to caries-active subjects. Urease levels were about 3-fold higher in the plaque of caries-free subjects (p < 0.0001). Although higher urease activity in saliva of caries-experienced and caries-free subjects was evident, no significant difference was found between the groups. Copyright (C) 2010 S. Karger AG, Basel