Multiple levels of interactions within the tetraspanin web
Multiple levels of interactions within the tetraspanin web
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DOI：
10.1016/s0006-291x(03)00545-x
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发表时间：
2003-04-25
影响因子：
3.1
通讯作者：
Rubinstein, E
中科院分区：
文献类型：
--
作者：
Charrin, S;Manié, S;Rubinstein, E
四联蛋白网是指四联蛋白和其他分子相互作用的网络。在四跨蛋白网内部，在特定条件下可以观察到仅含有一个四跨蛋白和一个特定伙伴分子（如CD151/alpha3beta1整合素和CD9/CD9P-1 (FPRP)）的小初级复合物。在这里，我们证明了当细胞被Brij97溶解时，四联蛋白cd151和CD9允许和/或稳定它们的伙伴分子与其他四联蛋白的相互作用，并且它们的两个伙伴分子在维持四联蛋白/四联蛋白相互作用的条件下结合。四跨蛋白也被发现分裂成耐洗涤剂的膜环境，整合素alpha3beta1在CD151表达后被重新定位。(C) 2003 Elsevier Science（美国）版权所有。
The tetraspanin web refers to a network of molecular interactions involving tetraspanins and other molecules. Inside the tetraspanin web, small primary complexes containing only one tetraspanin and one specific partner molecule such as CD151/alpha3beta1 integrin and CD9/CD9P-1 (FPRP) can be observed under particular conditions. Here we demonstrate that when cells are lysed with Brij97, the tetraspanins CD 151 and CD9 allow and/or stabilize the interaction of their partner molecules with other tetraspanins and that their two partners associate under conditions maintaining tetraspanin/tetraspanin interactions. The tetraspanins were also found to partition into a detergent-resistant membrane environment to which the integrin alpha3beta1 was relocalized upon expression of CD151. (C) 2003 Elsevier Science (USA). All rights reserved.