Heat shock protein hsp70 overexpression confers resistance against nitric oxide
Heat shock protein hsp70 overexpression confers resistance against nitric oxide
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DOI：
10.1016/0014-5793(96)00730-2
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发表时间：
1996-08-05
期刊：
影响因子：
3.5
通讯作者：
Kolb, H
中科院分区：
文献类型：
--
作者：
Bellmann, K;Jaattela, M;Kolb, H
热应激可使大鼠胰岛细胞抵抗一氧化氮、活性氧中间体和胰岛细胞毒素链脲佐菌素的毒性作用。本文首次报道了热休克蛋白70对一氧化氮的保护作用。热休克蛋白70的组成型表达对NO诱导的细胞裂解产生保护作用，其程度与热应激后细胞的保护作用相同。我们的研究结果证实热休克蛋白70是一种抗一氧化氮的防御分子。
Heat stress is known to render rat islet cells resistant against the toxic effects of nitric oxide, reactive oxygen intermediates and the islet cell toxin streptozotocin, We report here for the first time that protection against nitric oxide is mediated by the major heat shock protein, hsp70, even in the absence of heat stress, The human hsp70 gene was stably transfected into the rat insulinoma cell line RINm5F, Constitutive expression of hsp70 caused protection from NO-induced cell lysis which was of the same extent as seen after heat stressing cells, Our results identify hsp70 as a defence molecule against nitric oxide.